Department of Chemistry, Stanford University, Stanford, CA 94305, United States.
Department of Molecular Microbiology, Washington University, St. Louis, MO 63110, United States.
Biochem Biophys Res Commun. 2014 Jan 10;443(2):345-50. doi: 10.1016/j.bbrc.2013.11.026. Epub 2013 Nov 15.
Uropathogenic Escherichia coli (UPEC) are the major causative agents of urinary tract infection and engage in a coordinated genetic and molecular cascade to colonize the urinary tract. Disrupting the assembly and/or function of virulence factors and bacterial biofilms has emerged as an attractive target for the development of new therapeutic strategies to prevent and treat urinary tract infection, particularly in the era of increasing antibiotic resistance among human pathogens. UPEC vary widely in their genetic and molecular phenotypes and more data are needed to understand the features that distinguish isolates as more or less virulent and as more robust biofilm formers or poor biofilm formers. Curli are extracellular functional amyloid fibers produced by E. coli that contribute to pathogenesis and influence the host response during urinary tract infection (UTI). We have examined the production of curli and curli-associated phenotypes including biofilm formation among a specific panel of human clinical UPEC that has been studied extensively in the mouse model of UTI. Motility, curli production, and curli-associated biofilm formation attached to plastic were the most prevalent behaviors, shared by most clinical isolates. We discuss these results in the context on the previously reported behavior and phenotypes of these isolates in the murine cystitis model in vivo.
尿路致病性大肠杆菌（UPEC）是尿路感染的主要病原体，它们通过协调的遗传和分子级联反应定植于泌尿道。破坏毒力因子和细菌生物膜的组装和/或功能已成为开发新的治疗策略以预防和治疗尿路感染的有吸引力的目标，特别是在人类病原体对抗生素耐药性日益增加的时代。UPEC 在遗传和分子表型上差异很大，需要更多的数据来了解区分分离株的特征，这些特征使它们更具毒力，或者更能形成生物膜，或者更不能形成生物膜。卷曲菌是大肠杆菌产生的细胞外功能型淀粉样纤维，有助于发病机制，并在尿路感染（UTI）期间影响宿主反应。我们研究了特定的人类临床 UPEC 小组在尿路感染的小鼠模型中进行了广泛研究的卷曲菌的产生和卷曲菌相关表型，包括生物膜形成。运动性、卷曲菌的产生和附着在塑料上的卷曲菌相关生物膜形成是最常见的行为，大多数临床分离株都具有这些行为。我们将这些结果与这些分离株在体内小鼠膀胱炎模型中的先前报道的行为和表型进行了讨论。
