International Plant Research Institute, 94070, San Carlos, CA, USA.
Plant Cell Rep. 1983 Aug;2(4):209-12. doi: 10.1007/BF00270106.
This report describes the development of procedures for the production of somatic embryos in cell cultures of Glycine species including soybean. The conditions for callus induction and initiation of rapidly growing cell suspension cultures were defined. Methods for inducing embryogenesis were tested on 16 lines of several Glycine species and cultivars of soybean. The SB-26 Culture of a G. soja gave the best results and was used in the experiments. Embryogenesis required the presence of picloram or 2,4-D. AMO 1618, CCC, PP-333 and Ancymidol enhanced the embryogenesis frequency. Plants of the G. soja (SB-26) were grown to maturity from seed-derived shoot tips. Characteristics of the plants are discussed.
本报告描述了在 Glycine 物种（包括大豆）的细胞培养中生产体细胞胚的程序的开发。确定了愈伤组织诱导和快速生长细胞悬浮培养起始的条件。在几种 Glycine 物种和大豆品种的 16 个品系上测试了诱导胚胎发生的方法。G. soja 的 SB-26 培养物取得了最佳效果，并在实验中使用。胚胎发生需要 picloram 或 2,4-D 的存在。AMO 1618、CCC、PP-333 和 Ancymidol 提高了胚胎发生的频率。从种子衍生的茎尖生长出大豆（SB-26）的植株并成熟。讨论了植株的特征。
