Saeidnia Soodabeh, Permeh Parisa, Gohari Ahmad Reza, Mashinchian-Moradi Ali
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2012 Summer;11(3):845-9.
Gracilariopsis Persica (Rhodophyta) is one of the most abundant algae, introduced newly from the Indian Ocean. In this study, the main sterols of the algae have been isolated and identified. Separation and purification of the compounds was carried out on silica gel and sephadex LH20 column chromatography (CC) and high performance liquid chromatography (HPLC) to obtain five pure compounds 1-5. Structural elucidation of the compounds was based on the data obtained from H-NMR, (13)C-NMR, DEPT and Mass spectroscopy. The separated compounds from Gp. Persica were identified as 22-dehydrocholesterol (1), cholesterol (2), stigmasterol (3), β-sitosterol (4) and fucosterol (5) based on the spectral data compared to those reported in literatures. Most of these sterols are noteworthy for their effectiveness in decreasing the plasma cholesterol, glucose and inflammation. The results of Brine Shrimp Cytotoxicity Assay indicated that the ethyl acetate extract of Gp. Persica showed a high cytotoxic effect against A. salina nauplii (LC50 = 4 μg/mL). The methanol extract was no effective but the aqueous methanol extract was moderately effective (LC50 = 40 μg/mL) compared to berberine hydrochloride as a positive control (LC50 = 26 μg/mL).
波斯细基江蓠（红藻门）是从印度洋新引入的数量最为丰富的藻类之一。在本研究中，已对该藻类的主要甾醇进行了分离和鉴定。通过硅胶柱色谱、葡聚糖LH20柱色谱（CC）和高效液相色谱（HPLC）对化合物进行分离和纯化，以获得5种纯化合物1 - 5。化合物的结构解析基于从氢核磁共振（H-NMR）、碳核磁共振（¹³C-NMR）、无畸变极化转移增强（DEPT）和质谱获得的数据。与文献报道的数据相比，根据光谱数据，从波斯细基江蓠中分离出的化合物被鉴定为22-脱氢胆固醇（1）、胆固醇（2）、豆甾醇（3）、β-谷甾醇（4）和岩藻甾醇（5）。这些甾醇中的大多数因其在降低血浆胆固醇、血糖和炎症方面的有效性而值得关注。卤虫细胞毒性试验结果表明，波斯细基江蓠的乙酸乙酯提取物对卤虫无节幼体显示出高细胞毒性作用（半数致死浓度LC50 = 4 μg/mL）。与作为阳性对照的盐酸小檗碱（LC50 = 26 μg/mL）相比，甲醇提取物无效，但甲醇水溶液提取物有中等效果（LC50 = 40 μg/mL）。
