Department of Chemistry, Saitama University, Shimo-okubo 255, Sakura, Saitama 338-8570, Japan.
Chem Soc Rev. 2014 Apr 7;43(7):2147-58. doi: 10.1039/c3cs60226e. Epub 2013 Nov 19.
With the recent development in pulsed lasers with ultrashort pulse widths or wavelengths, spatially precise, low-damage processing by femtosecond or deep-UV laser ablation has shown promise for the production of protein single crystals suitable for X-ray crystallography. Femtosecond laser processing of supersaturated solutions can shorten the protein nucleation period or can induce nucleation at low supersaturation, which improves the crystal quality of various proteins including membrane proteins and supra-complexes. In addition to nucleation, processing of protein crystals by femtosecond or deep-UV laser ablation can produce single crystalline micro- or macro-seeds without deterioration of crystal quality. This tutorial review gives an overview of the successful application of laser ablation techniques to nucleation and seeding for the production of protein single crystals, and also describes the advantages from a physico-chemical perspective.
随着超短脉宽或波长脉冲激光器的最新发展，飞秒或深紫外激光烧蚀的空间精确、低损伤处理有望用于生产适合 X 射线晶体学的蛋白质单晶。过饱和溶液的飞秒激光处理可以缩短蛋白质成核周期，或者可以在低过饱和度下诱导成核，从而提高各种蛋白质（包括膜蛋白和超复合物）的晶体质量。除了成核之外，飞秒或深紫外激光烧蚀处理蛋白质晶体还可以在不降低晶体质量的情况下产生单晶微或宏观种子。本教程综述介绍了激光烧蚀技术在蛋白质单晶生产中成核和接种中的成功应用，并从物理化学的角度描述了其优势。
