Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel.
Theor Appl Genet. 1985 Sep;70(6):634-42. doi: 10.1007/BF00252289.
Several high molecular weight endosperm glutenin subunits, coded by genes located on chromosomes 1A, 1B and 1D of common wheat, Triticum aestivum L. em. Thell., were isolated from excised gel segments and subjected to amino acid analysis and peptide mapping; the latter was carried out following a limited digestion with trypsin, chymotrypsin or Staphylococcus aureus - V8 protease. Generally, all high molecular weight glutenins had a similar amino acid composition but several significant differences were observed in some of them. Both analyses revealed that the structural similarity among the various subunits was related to the homology of the genes coding them: subunits coded by homoalleles, i.e., different alleles of the same gene, were most similar; those coded by homoeoalleles, i.e., alleles of homoeologous genes, were less similar; whereas subunits coded either by alleles of different genes of the same gene cluster, or by nonhomoeoalleles of homoeologous clusters, were the least similar. Several small peptides derived from protease digestion of various subunits had a higher than expected staining intensity indicating that small peptide repeats may be interspersed within the glutenin subunits. The evolutionary course of the high molecular weight glutenins is discussed.
几种高分子量麦谷蛋白亚基，由普通小麦（Triticum aestivum L. em. Thell.）染色体 1A、1B 和 1D 上的基因编码，从小麦凝胶片段中分离出来，进行氨基酸分析和肽图谱分析；后者采用胰蛋白酶、糜蛋白酶或金黄色葡萄球菌 V8 蛋白酶进行有限消化。一般来说，所有高分子量麦谷蛋白都具有相似的氨基酸组成，但在其中一些蛋白中观察到了几个显著的差异。这两种分析都表明，各种亚基之间的结构相似性与编码它们的基因的同源性有关：由同等位基因编码的亚基，即同一基因的不同等位基因，最为相似；由同系等位基因编码的亚基，即同源基因的等位基因，不太相似；而由同一基因簇的不同基因的等位基因编码的亚基，或由同源基因簇的非同系等位基因编码的亚基，最为不相似。从不同亚基的蛋白酶消化中得到的几个小肽具有高于预期的染色强度，这表明小肽重复可能在麦谷蛋白亚基内间隔排列。讨论了高分子量麦谷蛋白的进化过程。
