China National Research Institute, Hongzhou, China.
Plant Cell Rep. 1984 Jun;3(3):88-90. doi: 10.1007/BF02441006.
A white, compact embryogenic tissue was obtained from young inflorescence segments ofEchinochloa crusgalli (barnyard grass) cultured on Murashige and Skoog's medium containing various concentrations and combinations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. Histological and scanning electron microscopic studies revealed that the white compact callus contained embryoids in various stages of development. Typical embryoids were bipolar and possessed scutella, coleoptiles and coleorhizae. The embryogenic nature of the callus was maintained throughout eight to ten subcultures spanning more than six months. A high frequency of plant regeneration was obtained when the 2,4-D concentration was reduced or 2,4-D was removed from the medium.
从培养在含有不同浓度和组合的 2,4-二氯苯氧乙酸和 6-苄基氨基嘌呤的 Murashige 和 Skoog 培养基上的稗草幼花序节上获得了白色紧凑的胚性组织。组织学和扫描电子显微镜研究表明，白色紧凑的愈伤组织中含有不同发育阶段的胚状体。典型的胚状体为双极，具有盾片、胚芽鞘和下胚轴。愈伤组织的胚性在超过六个月的八到十次继代培养中得以维持。当降低 2,4-D 的浓度或从培养基中去除 2,4-D 时，获得了高频率的植物再生。
