过氧化物酶 I 活性与曼陀罗愈伤组织培养中的细胞增殖的相关性。
Correlation of glyoxalase I activity with cell proliferation inDatura callus culture.
机构信息
Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India.
出版信息
Plant Cell Rep. 1984 Jun;3(3):121-4. doi: 10.1007/BF02441015.
Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent and 50 percent respectively, at the most effective concentration and the callus growth was also reduced. Similar results were obtained with specific glyoxalase I inhibitors, iso-ascorbate and squaric acid.
在生长中的曼陀罗愈伤组织中，甘油醛酶-I 的活性随培养时间的延长增加了 184%。亚精胺与 DNA 和蛋白质合成一起增加了酶的活性。在生长培养基中添加有丝分裂抑制剂长春花碱和甲基乙二醛，在最有效浓度时，酶活性分别被抑制了 92%和 50%，同时愈伤组织的生长也减少了。特异性甘油醛酶 I 抑制剂异抗坏血酸和戊烯二酸也得到了类似的结果。
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