Gee C E, Griffin J, Sastre L, Miller L J, Springer T A, Piwnica-Worms H, Roberts T M
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5131-5. doi: 10.1073/pnas.83.14.5131.
We have detected a significant increase in the levels of pp60c-src kinase activity associated with the differentiation of myeloid cell lines HL-60 and U-937. The induction of pp60c-src kinase activity becomes apparent approximately 14 hr after the addition of phorbol 12-myristate 13-acetate and increases 20-fold by 72 hr. The enhanced kinase activity can be accounted for by elevated levels of c-src protein in the differentiated cells. When nonleukemic bone marrow cells were examined, myeloid progenitor cells exhibited a low level of pp60c-src kinase activity. As these cells are allowed to differentiate in culture, the resulting adherent monocytes are as high in pp60c-src kinase activity as HL-60 cells induced to differentiate into monocytes. A strong correlation is found between the levels of pp60c-src kinase activity and the degree of monocytic differentiation of the cells from patients with acute myeloid leukemia. Our findings suggest that the activation of pp60c-src kinase activity is a normal physiological event associated with myeloid differentiation.
我们检测到与髓系细胞系HL-60和U-937分化相关的pp60c-src激酶活性水平显著增加。在添加佛波醇12-肉豆蔻酸酯13-乙酸酯后约14小时，pp60c-src激酶活性的诱导变得明显，并在72小时内增加20倍。分化细胞中c-src蛋白水平升高可解释激酶活性增强。当检测非白血病骨髓细胞时，髓系祖细胞表现出低水平的pp60c-src激酶活性。随着这些细胞在培养中分化，产生的贴壁单核细胞的pp60c-src激酶活性与诱导分化为单核细胞的HL-60细胞一样高。在急性髓系白血病患者的细胞中，pp60c-src激酶活性水平与单核细胞分化程度之间存在很强的相关性。我们的研究结果表明，pp60c-src激酶活性的激活是与髓系分化相关的正常生理事件。
