Guo Ling, Yang Xiangdong, Xia Qing, Zhen Junhui, Zhuang Xuewei, Peng Tao
Department of Nephrology, Shandong University Qilu Hospital, Jinan, 250012, China.
Clin Exp Med. 2014 Nov;14(4):383-8. doi: 10.1007/s10238-013-0264-3. Epub 2013 Nov 20.
The aim of this study was to evaluate the expression of TIM-3 in renal tissue from patients with systemic lupus erythematosus (SLE) and patients without SLE, and to evaluate the difference of TIM-3 expression between them. A total of 272 patients with SLE as SLE group and 62 patients without SLE as control group were enrolled in the present study. Patients with SLE accepted percutaneous renal biopsy. We examined the expression of TIM-3 in renal tissue and the serological parameters in serum from all enrolled cases. The expression of TIM-3 and serological parameters were compared between the different groups. Positive staining of TIM-3 protein was seen in 97.1 % patients with SLE (264 out of 272), but 95.2 % negative staining in the cases without SLE (59 out of 62), only 3 out of 62 patients in control group were positive staining of TIM-3. There were significant differences between two groups in almost all serological markers which reflect SLE activity. There was a nearly positive correlation between pathological manifestations and expression degree of TIM-3. High immuno-reactivity of TIM-3 was found to be significantly correlated with serological grade (p < 0.001), but there was the phenomenon in control group. The results showed that there was the expression of TIM-3 in renal tissue from the patients with SLE, but rarely expression in cases without SLE. Expression of TIM-3 was associated with the diseases' activity.
本研究旨在评估系统性红斑狼疮（SLE）患者与非SLE患者肾组织中TIM-3的表达情况，并评估两者之间TIM-3表达的差异。本研究共纳入272例SLE患者作为SLE组，62例非SLE患者作为对照组。SLE患者接受经皮肾活检。我们检测了所有纳入病例肾组织中TIM-3的表达及血清中的血清学参数。比较了不同组之间TIM-3的表达及血清学参数。97.1%的SLE患者（272例中的264例）TIM-3蛋白呈阳性染色，而在非SLE病例中95.2%呈阴性染色（62例中的59例），对照组62例患者中仅有3例TIM-3呈阳性染色。几乎所有反映SLE活动的血清学标志物在两组之间均存在显著差异。病理表现与TIM-3表达程度之间存在近乎正相关。发现TIM-3的高免疫反应性与血清学分级显著相关（p < 0.001），但对照组也存在这种现象。结果表明，SLE患者肾组织中有TIM-3表达，而非SLE病例中很少表达。TIM-3的表达与疾病活动相关。
