Marone G, Columbo M, de Paulis A, Cirillo R, Giugliano R, Condorelli M
Agents Actions. 1986 Apr;18(1-2):103-6. doi: 10.1007/BF01987995.
We have previously shown that physiological concentrations of zinc (congruent to 7 X 10(-6) M) inhibit the release of histamine from human basophil leukocytes (Marone et al., J. Pharmacol. Exp. Ther. 217: 292, 1981). In these experiments we compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung. Preincubation (5 min, 37 degrees C) of human basophils and lung mast cells with zinc chloride (10(-6)-3 X 10(-5) M) caused dose-related inhibition of histamine and peptide leukotriene C4 (LTC4) release induced by anti-IgE. Increase Ca2+ concentrations (0.3 to 6 mM) in the extracellular medium completely reversed the inhibitory effect of zinc on anti-IgE-mediated histamine secretion. Zinc chloride was a competitive antagonist of the action of Ca2+ in histamine secretion induced by anti-IgE with a dissociation constant (Kd) of about 10(-5) M in both the basophil and mast cell systems. Thus physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells, presumably by blocking Ca2+ uptake induced by anti-IgE activation.
我们之前已经表明，生理浓度的锌（约7×10⁻⁶ M）可抑制人嗜碱性粒细胞释放组胺（马罗内等人，《药理学与实验治疗学杂志》217: 292, 1981）。在这些实验中，我们比较了氯化锌对从人肺中分离出的人嗜碱性粒细胞和肥大细胞释放化学介质的影响。用氯化锌（10⁻⁶ - 3×10⁻⁵ M）对人嗜碱性粒细胞和肺肥大细胞进行预孵育（5分钟，37℃），导致抗IgE诱导的组胺和肽白三烯C4（LTC4）释放呈剂量依赖性抑制。细胞外培养基中钙离子浓度增加（0.3至6 mM）完全逆转了锌对抗IgE介导的组胺分泌的抑制作用。在嗜碱性粒细胞和肥大细胞系统中，氯化锌是抗IgE诱导的组胺分泌中钙离子作用的竞争性拮抗剂，解离常数（Kd）约为10⁻⁵ M。因此，生理浓度的锌可能通过阻断抗IgE激活诱导的钙离子摄取来抑制人嗜碱性粒细胞和肺肥大细胞释放组胺。
