Qureshi Tahira, Memon Najma, Memon Saima Q, Abro Kamran, Shah Syed Waliullah
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan.
Springerplus. 2013 Oct 29;2:575. doi: 10.1186/2193-1801-2-575. eCollection 2013.
Cephalosporins type antibiotics are widely used to treat infectious diseases. Their determination is not only important in blood/serum of patients under treatment but also in diverse matrices like wastewaters, milk etc. as contaminant. Keeping in view the need, a new high performance liquid chromatographic method for the determination of three cephalosporins (cefradine, cefuroxime and cefotaxime) has been developed. Separation was performed on an ODS column with binary solvent elution of aqueous formic acid (0.05%) and methanol in the ratio of 45: 55 (v/v) at a flow rate of 1 mL min(-1) and UV detection at 260 nm. Under optimised conditions, all three cephalosporins were baseline separated within 5 min. Linear responses for cefradine 5-20 μg mL(-1), cefuroxime 0.5-15 μg mL(-1) and cefotaxime 1.0-20 μg mL(-1) were established. LOD of 0.05-0.25 μg mL(-1) after preconcentration was achieved. The method was applied to serum samples of patients under treatment with these antibiotics and to screen the selected cephalosporins from hospital wastewater and milk samples. Moreover, method was applied to study stability of aqueous solutions and acid/base induced degradation of all three drugs.
头孢菌素类抗生素被广泛用于治疗传染病。对它们的测定不仅在接受治疗的患者的血液/血清中很重要，而且在废水、牛奶等各种基质中作为污染物的测定也很重要。考虑到这种需求，已开发出一种用于测定三种头孢菌素（头孢拉定、头孢呋辛和头孢噻肟）的新型高效液相色谱法。在ODS柱上进行分离，以体积比为45:55（v/v）的0.05%甲酸水溶液和甲醇进行二元溶剂洗脱，流速为1 mL min⁻¹，在260 nm处进行紫外检测。在优化条件下，所有三种头孢菌素在5分钟内实现基线分离。建立了头孢拉定5 - 20 μg mL⁻¹、头孢呋辛0.5 - 15 μg mL⁻¹和头孢噻肟1.0 - 20 μg mL⁻¹的线性响应。预浓缩后实现了0.05 - 0.25 μg mL⁻¹的检测限。该方法应用于接受这些抗生素治疗的患者的血清样本，以及从医院废水和牛奶样本中筛选选定的头孢菌素。此外，该方法还应用于研究所有三种药物水溶液的稳定性以及酸碱诱导的降解。
