Zhao Shoujing, Wang Le, Liu Li, Liang Yanlong, Sun Yao, Wu Jianjun
College of Biological and Agricultural Engineering, Jilin University, Changchun, 130022, China.
Plant Cell Rep. 2014 Mar;33(3):393-400. doi: 10.1007/s00299-013-1538-7. Epub 2013 Nov 19.
When one of them was inhibited, the two pathways could compensate with each other to guarantee normal growth. Moreover, the sterol biosynthesis inhibitor miconazole could enhance ginsenoside level.
Ginsenosides, a kind of triterpenoid saponins derived from isopentenyl pyrophosphate (IPP), represent the main pharmacologically active constituents of ginseng. In plants, two pathways contribute to IPP biosynthesis, namely, the mevalonate pathway in cytosol and the non-mevalonate pathway in plastids. This motivates biologists to clarify the roles of the two pathways in biosynthesis of IPP-derived compounds. Here, we demonstrated that both pathways are involved in ginsenoside biosynthesis, based on the analysis of the effects from suppressing either or both of the pathways on ginsenoside accumulation in Panax ginseng hairy roots with mevinolin and fosmidomycin as specific inhibitors for the mevalonate and the non-mevalonate pathways, respectively. Furthermore, the sterol biosynthesis inhibitor miconazole could enhance ginsenoside levels in the hairy roots. These results shed some light on the way toward better understanding of ginsenoside biosynthesis.
当其中一条途径被抑制时，两条途径可以相互补偿以保证正常生长。此外，甾醇生物合成抑制剂咪康唑可以提高人参皂苷水平。
人参皂苷是一种由异戊烯基焦磷酸（IPP）衍生而来的三萜皂苷，是人参的主要药理活性成分。在植物中，有两条途径参与IPP的生物合成，即细胞质中的甲羟戊酸途径和质体中的非甲羟戊酸途径。这促使生物学家阐明这两条途径在IPP衍生化合物生物合成中的作用。在此，我们分别以美伐他汀和磷霉素作为甲羟戊酸途径和非甲羟戊酸途径的特异性抑制剂，通过分析抑制人参发根中一条或两条途径对人参皂苷积累的影响，证明了这两条途径均参与人参皂苷的生物合成。此外，甾醇生物合成抑制剂咪康唑可以提高发根中的人参皂苷水平。这些结果为更好地理解人参皂苷生物合成的途径提供了一些线索。
