• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

烟草叶绿体基因组：在大肠杆菌和枯草芽孢杆菌中表达烟草和衣藻叶绿体 DNA 序列编码的 RuBP 羧化酶大亚基。

Nicotiana chloroplast genome : 7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase.

机构信息

Department of Biological Sciences, University of Maryland, Baltimore County, 5401 Wilkens Avenue, 21228, Catonsville, MD, USA.

出版信息

Theor Appl Genet. 1984 Feb;67(4):333-6. doi: 10.1007/BF00272870.

DOI:10.1007/BF00272870
PMID:24258655
Abstract

RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.

摘要

核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBPCase），是一种在多种光合生物中负责催化 RuBP 羧化和氧化的酶，是叶绿体中发现的主要蛋白质。本文首次提供了烟草和衣藻 ct-DNA 序列在大肠杆菌和枯草芽孢杆菌中直接表达的证据，通过简单的原位免疫测定证实了这一点。

相似文献

1
Nicotiana chloroplast genome : 7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase.烟草叶绿体基因组：在大肠杆菌和枯草芽孢杆菌中表达烟草和衣藻叶绿体 DNA 序列编码的 RuBP 羧化酶大亚基。
Theor Appl Genet. 1984 Feb;67(4):333-6. doi: 10.1007/BF00272870.
2
The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.烟草叶绿体中核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基基因的核苷酸序列。
Gene. 1982 Nov;20(1):91-102. doi: 10.1016/0378-1119(82)90090-7.
3
Identification and cloning of the chloroplast gene coding for the large subunit of ribulose-1,5-bisphosphate carboxylase from Chlamydomonas reinhardi.莱茵衣藻中编码1,5-二磷酸核酮糖羧化酶大亚基的叶绿体基因的鉴定与克隆。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3193-7. doi: 10.1073/pnas.74.8.3193.
4
The relationship between carbon-dioxide-limited photosynthetic rate and ribulose-1,5-bisphosphate-carboxylase content in two nuclear-cytoplasm substitution lines of wheat, and the coordination of ribulose-bisphosphate-carboxylation and electron-transport capacities.小麦两种核质替换系中二氧化碳限制光合速率与核酮糖-1,5-二磷酸羧化酶含量的关系以及核酮糖-1,5-二磷酸羧化与电子传递能力的协调。
Planta. 1986 Mar;167(3):351-8. doi: 10.1007/BF00391338.
5
Regulation of genes encoding the large subunit of ribulose-1,5-bisphosphate carboxylase and the photosystem II polypeptides D-1 and D-2 during the cell cycle of Chlamydomonas reinhardtii.莱茵衣藻细胞周期中编码1,5-二磷酸核酮糖羧化酶大亚基以及光系统II多肽D-1和D-2的基因调控
J Cell Biol. 1986 Nov;103(5):1837-45. doi: 10.1083/jcb.103.5.1837.
6
Specific reduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase activity by antisense RNA reduces CO2 assimilation via a reduction in ribulose bisphosphate regeneration in transgenic tobacco plants.通过反义RNA特异性降低叶绿体甘油醛-3-磷酸脱氢酶活性，会因转基因烟草植株中核酮糖二磷酸再生减少而降低二氧化碳同化作用。
Planta. 1995;195(3):369-78. doi: 10.1007/BF00202594.
7
The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii.在莱茵衣藻中，RuBP 羧化酶/加氧酶小亚基的两个基因紧密连锁。
Plant Mol Biol. 1986 Jan;6(1):13-21. doi: 10.1007/BF00021302.
8
The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence.纤细裸藻叶绿体DNA中1,5 - 二磷酸核酮糖羧化酶大亚基的基因：定位、极性、克隆及存在间隔序列的证据
Nucleic Acids Res. 1982 Jun 11;10(11):3427-44. doi: 10.1093/nar/10.11.3427.
9
Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA.玉米线粒体DNA含有一段与叶绿体DNA中核酮糖-1,5-二磷酸羧化酶大亚基基因同源的序列。
Cell. 1983 Oct;34(3):1007-14. doi: 10.1016/0092-8674(83)90558-5.
10
Chlorella chloroplast DNA sequence containing a gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and a part of a possible gene for the β' subunit of RNA polymerase.衣藻叶绿体 DNA 序列含有编码核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基的基因和 RNA 聚合酶β'亚基可能的基因的一部分。
Plant Mol Biol. 1988 May;10(3):245-50. doi: 10.1007/BF00027401.

引用本文的文献

1
Synthesis and assembly of bacterial and higher plant Rubisco subunits in Escherichia coli.在大肠杆菌中细菌和高等植物 Rubisco 亚基的合成和组装。
Photosynth Res. 1988 Jul;17(1-2):145-57. doi: 10.1007/BF00047686.
2
Using bacteria to analyze sequences involved in chloroplast gene expression.利用细菌分析参与叶绿体基因表达的序列。
Photosynth Res. 1988 Jan;19(1-2):7-22. doi: 10.1007/BF00114566.
3
Comparative restriction endonuclease analysis and molecular cloning of plastid DNAs from wild species and cultivated varieties of the genus Beta (L.).

本文引用的文献

1
Cloning, mapping, and in vitro transcription-translation of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplasts.菠菜叶绿体 RuBP 羧化酶大亚基基因的克隆、定位和体外转录-翻译。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3459-63. doi: 10.1073/pnas.78.6.3459.
2
Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase.1,5-二磷酸核酮糖羧化酶小亚基前体向完整叶绿体的翻译后转运
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6110-4. doi: 10.1073/pnas.75.12.6110.
3
Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase.
比较限制内切酶分析和分子克隆野生种和栽培种的贝塔属（L.）叶绿体 DNA。
Theor Appl Genet. 1987 Sep;74(5):589-94. doi: 10.1007/BF00288857.
编码核酮糖二磷酸羧化酶大亚基的玉米叶绿体DNA片段。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487-91. doi: 10.1073/pnas.74.12.5487.
4
Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase.玉米和小麦叶绿体中核酮糖二磷酸羧化酶大亚基基因在大肠杆菌中的表达
Nature. 1981 May 14;291(5811):117-21. doi: 10.1038/291117a0.
5
Expression of Escherichia coli trp genes and the mouse dihydrofolate reductase gene cloned in Bacillus subtilis.克隆于枯草芽孢杆菌中的大肠杆菌色氨酸基因和小鼠二氢叶酸还原酶基因的表达。
Gene. 1981 Dec;16(1-3):199-206. doi: 10.1016/0378-1119(81)90076-7.
6
Chloroplast deoxyribonucleic acid isolation: purity achieved without nuclease digestion.叶绿体脱氧核糖核酸的分离：无需核酸酶消化即可实现纯度
Can J Biochem. 1981 Nov-Dec;59(11-12):911-5. doi: 10.1139/o81-128.
7
Construction of a SalI/PstI restriction map of spinach chloroplast DNA using low-gelling-temperature-agarose electrophoresis.利用低熔点琼脂糖凝胶电泳构建菠菜叶绿体DNA的SalI/PstI限制酶切图谱。
Gene. 1980 Jan;8(2):179-91. doi: 10.1016/0378-1119(80)90036-0.
8
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.DNA和RNA向硝酸纤维素或重氮苄氧基甲基纸的双向转移。
Anal Biochem. 1980 Nov 15;109(1):123-9. doi: 10.1016/0003-2697(80)90019-6.
9
Chloroplast DNA codes for the primary structure of the large subunit of fraction I protein.叶绿体DNA编码I类蛋白大亚基的一级结构。
Biochim Biophys Acta. 1972 Sep 14;277(3):677-80. doi: 10.1016/0005-2787(72)90115-3.
10
Synthesis of the small subunit of ribulose 1,5-diphosphate carboxylase on cytoplasmic ribosomes from greening bean leaves.来自绿豆叶片的细胞质核糖体上1,5-二磷酸核酮糖羧化酶小亚基的合成。
FEBS Lett. 1973 Dec 15;38(1):67-9. doi: 10.1016/0014-5793(73)80515-0.