烟草叶绿体基因组：在大肠杆菌和枯草芽孢杆菌中表达烟草和衣藻叶绿体 DNA 序列编码的 RuBP 羧化酶大亚基。
Nicotiana chloroplast genome : 7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase.
机构信息
Department of Biological Sciences, University of Maryland, Baltimore County, 5401 Wilkens Avenue, 21228, Catonsville, MD, USA.
出版信息
Theor Appl Genet. 1984 Feb;67(4):333-6. doi: 10.1007/BF00272870.
PMID:24258655
Abstract
RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.
摘要
核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBPCase），是一种在多种光合生物中负责催化 RuBP 羧化和氧化的酶，是叶绿体中发现的主要蛋白质。本文首次提供了烟草和衣藻 ct-DNA 序列在大肠杆菌和枯草芽孢杆菌中直接表达的证据，通过简单的原位免疫测定证实了这一点。
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