Gatenby A A, Castleton J A, Saul M W
Nature. 1981 May 14;291(5811):117-21. doi: 10.1038/291117a0.
Synthesis of the large subunit polypeptide of ribulose bisphosphate carboxylase can be detected in Escherichia coli cells containing the chloroplast genes from maize and wheat. The chloroplast DNA sequences contain a 'promoter' that, in Escherichia coli, initiates transcription and translation of the genes. This synthesis of large subunits has been substantially increased using the powerful lambda promoter, PL, to drive transcription of the maize gene.
在含有来自玉米和小麦叶绿体基因的大肠杆菌细胞中,可以检测到核酮糖二磷酸羧化酶大亚基多肽的合成。叶绿体DNA序列包含一个“启动子”,在大肠杆菌中,该启动子可启动这些基因的转录和翻译。利用强大的λ启动子PL来驱动玉米基因的转录,大亚基的这种合成已大幅增加。