Kang Wenyu, Zhang Fang, Su Yue, Guo Yinlong
Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Eur J Mass Spectrom (Chichester). 2013;19(2):103-10. doi: 10.1255/ejms.1218.
A post-target analysis method based on gas chromatography coupled to a high-resolution quadrupole time-of-flight mass analyzer is applied for the investigation of volatile compounds in Fructus Amomi. A series of narrow window extracted ion chromatograms at selected characteristic ions were performed. Chromatographic peaks with the same retention time in different extracted ion chromatograms was used to screen out the candidate compound. Identification was achieved by the accurate masses of several characteristic ions and the retention index of the peak. Forty six compounds, including 12 monoterpene compounds, were identified by conventional static headspace gas chromatography mass spectrometry and another six monoterpene compounds were found and identified by the post-target method. Post-target analysis is a useful strategy in qualitative research of natural products.
一种基于气相色谱与高分辨率四极杆飞行时间质谱仪联用的靶向分析后方法被应用于砂仁中挥发性化合物的研究。在选定的特征离子处进行了一系列窄窗口提取离子色谱图分析。利用不同提取离子色谱图中保留时间相同的色谱峰筛选出候选化合物。通过几个特征离子的精确质量和峰的保留指数进行鉴定。通过常规静态顶空气相色谱质谱法鉴定出46种化合物，包括12种单萜类化合物，另外通过靶向分析后方法又发现并鉴定出6种单萜类化合物。靶向分析后方法是天然产物定性研究中的一种有用策略。
