Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi 221005, India.
Ecotoxicol Environ Saf. 2014 Feb;100:178-87. doi: 10.1016/j.ecoenv.2013.10.032. Epub 2013 Nov 21.
Increase in perception of solar ultraviolet-B (UV-B) radiation on Earth's surface due to anthropogenic activities has potential in causing detrimental effects on plants. The present study was performed to evaluate the effect of elevated UV-B on Pisum sativum L., a leguminous plant with emphasis on nitrogen metabolism, flavonoids and hormonal changes. Elevated UV-B (ambient+7.2 kJ m(-2) day(-1)) negatively affected the growth, biomass, yield and its quality by generating oxidative stress directly or due to elevation of salicylic acid in two cultivars with higher magnitude being observed in HUP-2 as compared to HUDP-15. The increased accumulation of flavonoids (quercetin and kaempferol) under elevated UV-B neither provided sufficient protection to the photosynthetic machinery nor helped in elevation of biological nitrogen fixation. Nitrogen fixation and its assimilation were negatively affected under elevated UV-B as observed by the decline in nitrogenase, nitrate reductase, nitrite reductase activities and leghaemoglobin contents. Higher accumulation of salicylic acid in HUP-2 might be associated with its higher degree of sensitivity against UV-B, while higher induction of jasmonic acid and antioxidative enzymes (superoxide dismutase, catalase and ascorbate peroxidase activities) provided resistance to HUDP-15 against applied stress vis-a-vis exhibited less reduction in biomass, yield and quality of produce.
由于人为活动，地球表面感知到的太阳紫外线-B（UV-B）辐射增加，有可能对植物造成有害影响。本研究旨在评估升高的 UV-B 对豆科植物豌豆（Pisum sativum L.）的影响，重点关注氮代谢、类黄酮和激素变化。升高的 UV-B（环境+7.2 kJ m(-2) day(-1)）通过直接产生氧化应激或由于水杨酸的升高，对两种品种的生长、生物量、产量及其质量产生负面影响，其中 HUP-2 比 HUDP-15 观察到的影响更大。在升高的 UV-B 下，类黄酮（槲皮素和山奈酚）的积累增加既不能为光合作用机制提供足够的保护，也不能帮助提高生物固氮作用。氮固定及其同化作用受到升高的 UV-B 的负面影响，如氮酶、硝酸还原酶、亚硝酸盐还原酶活性和豆血红蛋白含量的下降所观察到的那样。在 HUP-2 中水杨酸的更高积累可能与其对 UV-B 的更高敏感度有关，而茉莉酸和抗氧化酶（超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶活性）的更高诱导为 HUDP-15 提供了对施加应激的抗性，相对于表现出较少的生物量、产量和产量减少而言。
