Department of Oral and Maxillofacial Surgery, School of Stomatology, PekingUniversity, Beijing, China.
Histopathology. 2014 Feb;64(3):348-55. doi: 10.1111/his.12258. Epub 2013 Nov 26.
In this study, we sought to determine the prognostic significance of glycerol-3-phosphate dehydrogenase 1-like (GPD1L) expression in head and neck squamous cell carcinoma (HNSCC).
The mRNA levels of GPD1L were measured in 70 paired HNSCC and corresponding adjacent normal tissues using real-time PCR. GPD1L protein levels were evaluated in HNSCC from 135 patients using immunohistochemical staining. Correlations were analysed between GPD1L levels and local recurrence rate, regional recurrence rate, second primary malignancy rate), disease-free survival (DFS) and disease-specific survival (DSS). The results of real-time PCR showed that, compared with the paired normal tissues, mRNA levels of GPD1L were decreased significantly in HNSCC (P < 0.001). Patients whose tumours showed high GPD1L protein expression had a significantly better prognosis than those whose tumours showed low expression (61.3% versus 21.4%, P < 0.001 for DFS; 68% versus 39.3%, P = 0.001 for DSS). High GPD1L expression was associated with a lower local recurrence rate than low GPD1L expression (P = 0.049). Multivariate survival analysis also showed that GPD1L expression was an independent prognostic factor (P = 0.001).
Our results indicate that the GPD1L expression is a strong predictor for local recurrence and survival in HNSCC.
本研究旨在探讨甘油-3-磷酸脱氢酶 1 样蛋白（GPD1L）在头颈部鳞状细胞癌（HNSCC）中的表达与预后的关系。
采用实时定量 PCR 检测 70 对 HNSCC 组织及其相应的癌旁正常组织中 GPD1L 的 mRNA 水平，采用免疫组织化学染色法检测 135 例 HNSCC 组织中 GPD1L 蛋白的表达水平。分析 GPD1L 水平与局部复发率、区域复发率、第二原发恶性肿瘤率、无病生存率（DFS）和疾病特异性生存率（DSS）之间的相关性。实时 PCR 结果显示，与配对的正常组织相比，GPD1L 在 HNSCC 中的 mRNA 水平显著降低（P<0.001）。GPD1L 蛋白高表达的患者预后明显优于低表达的患者（DFS：61.3%比 21.4%，P<0.001；DSS：68%比 39.3%，P=0.001）。GPD1L 高表达与局部复发率较低相关（P=0.049）。多因素生存分析也表明 GPD1L 表达是独立的预后因素（P=0.001）。
本研究结果表明，GPD1L 表达是 HNSCC 局部复发和生存的一个强有力的预测指标。
