Yang Yang, Jin Tiefeng, Liu Shuangping, Chen Liyan, Lin Lijuan, Han Hongxiu, Li Longshan, Lin Zhenhua
Department of Pathology, Yanbian University Medical College, Yanji 133002, China.
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Zhonghua Bing Li Xue Za Zhi. 2014 Jul;43(7):463-7.
To investigate the significance of NADPH quinine oxidoreductase 1 (NQO1) protein overexpression on prognostic evaluation of head and neck squamous cell carcinoma (HNSCC).
NQO1 protein was detected in 162 of HNSCC, 45 cases of adjacent nontumor tissues and 26 samples of normal head and neck epithelia using EnVision immunohistochemical. Correlation between NQO1 overexpression and patients prognosis was also analyzed.
The positive rate and strongly positive rate of NQO1 protein were 84.0% (136/162) and 69.8% (113/162) in HNSCC, respectively, and both of which were significantly higher than either those in adjacent nontumor tissues and normal head and neck epithelia (both P < 0.01). NQO1 expression was significantly correlated with the clinical stage, pT and chemoradiotherapy of HNSCC (P < 0.01). Kaplan-Meier survival analysis showed that overall survival and disease-free survival rates were significantly higher in HNSCC patients with high level NQO1 expression than that those with low level of NQO1 expression (Log-rank = 6.625 , P = 0.010;Log-rank = 6.234 , P = 0.013). Additional analysis by Cox proportional hazard regression model showed that high level of NQO1 expression was an independent hazard predictor for overall survival of patients with HNSCC (Wald = 6.626, P = 0.008).
NQO1 expression level is closely correlated with the progression and prognosis of patients with HNSCC. High level of NQO1 expression may be used as an important indicator for patients with poor prognostic HNSCC.
探讨烟酰胺腺嘌呤二核苷酸磷酸醌氧化还原酶1（NQO1）蛋白过表达在头颈部鳞状细胞癌（HNSCC）预后评估中的意义。
采用EnVision免疫组织化学法检测162例HNSCC组织、45例癌旁非肿瘤组织及26例正常头颈部上皮组织中的NQO1蛋白，并分析NQO1过表达与患者预后的相关性。
HNSCC组织中NQO1蛋白阳性率和强阳性率分别为84.0%（136/162）和69.8%（113/162），均显著高于癌旁非肿瘤组织和正常头颈部上皮组织（均P<0.01）。NQO1表达与HNSCC的临床分期、pT及放化疗显著相关（P<0.01）。Kaplan-Meier生存分析显示，NQO1表达水平高的HNSCC患者总生存率和无病生存率显著高于NQO1表达水平低的患者（Log-rank = 6.625，P = 0.010；Log-rank = 6.234，P = 0.013）。Cox比例风险回归模型进一步分析显示，NQO1高表达是HNSCC患者总生存的独立危险因素（Wald = 6.626，P = 0.008）。
NQO1表达水平与HNSCC患者的病情进展及预后密切相关，NQO1高表达可能作为HNSCC预后不良患者的重要指标。
