Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, T6G 2P5, Canada.
Food Funct. 2014 Jan;5(1):92-101. doi: 10.1039/c3fo60351b.
This is the first report in which barley protein nanoparticles were prepared with the aim of developing a delivery system for lipophilic bioactive compounds at ambient temperature using high pressure homogenization. No organic solvents or crosslinking reagents were involved in the nanoparticle preparation. Effects of processing conditions and formulae on particle size and size distribution were investigated. Optimal nanoparticles with regular spherical shape, small size (90-150 nm) and narrow size distribution (PDI < 0.3) could be achieved at a protein weight concentration of up to 5% when the oil/protein ratio was maintained within a range of 1 to 1.5. These nanoparticles exhibited high zeta-potential (about -35 mV), high payload (51.4-54.5%) and good stability without the use of surfactants. As shown by the release test, though the bulk protein matrices of nanoparticles were degraded in the simulated gastric tract, even smaller nanoparticles were released and bioactive compounds were protected by a layer of barley protein. Then, complete release occurred in the simulated intestinal environments due to pancreatin degradation. In vitro studies showed that barley protein nanoparticles are relatively safe and could be internalized by Caco-2 cells and accumulated in the cytoplasm.
这是第一篇报道，其中使用高压匀浆法制备大麦蛋白纳米颗粒，目的是开发亲脂性生物活性化合物在环境温度下的递送系统。纳米颗粒的制备过程中不涉及有机溶剂或交联试剂。研究了加工条件和配方对粒径和粒径分布的影响。当油/蛋白比保持在 1 到 1.5 的范围内时，在蛋白质重量浓度高达 5%的情况下，可以得到具有规则球形、小粒径（90-150nm）和窄粒径分布（PDI<0.3）的最佳纳米颗粒。这些纳米颗粒具有高 Zeta 电位（约-35mV）、高载药量（51.4-54.5%）和良好的稳定性，无需使用表面活性剂。如释放试验所示，尽管纳米颗粒的蛋白质基质在模拟胃液中降解，但即使更小的纳米颗粒也会释放出来，并且生物活性化合物受到大麦蛋白层的保护。然后，由于胰酶降解，在模拟肠道环境中完全释放。体外研究表明，大麦蛋白纳米颗粒相对安全，可以被 Caco-2 细胞内化，并在细胞质中积累。
