Richert J R, Rose J W, Reuben-Burnside C, Kearns M C, Jacobson S, Mingioli E S, Hartzman R J, McFarland H F, McFarlin D E
J Immunol. 1986 Oct 1;137(7):2190-4.
Eleven cloned and uncloned measles virus-specific T cell lines were generated from peripheral blood lymphocytes obtained from a patient with multiple sclerosis and were assayed for measles polypeptide specificity. Three clones reacted specifically with the fusion (F) protein and one recognized the hemagglutinin (HA). Two reacted with whole virus but not with any of the purified proteins. Five cell lines proliferated in response to multiple measles polypeptides. The addition of anti-HA or anti-F monoclonal antibodies to two of the multispecific cell lines each resulted in partial suppression of the proliferative response to whole virus by the cell lines. Two of the three F-reactive clones recognized antigen in association with a subgroup of HLA-DR4; the third responded to F only in the presence of autologous antigen-presenting cells. Of the two clones that reacted only with whole virus, one was restricted to DP3 and one to autologous cells. The HA-specific clone was DP3 restricted. Several cell lines recognized multiple measles polypeptides in association with a single HLA antigen. Recognition of individual measles polypeptides does not segregate with specific genetic restriction elements.
从一名多发性硬化症患者的外周血淋巴细胞中产生了11个克隆和未克隆的麻疹病毒特异性T细胞系，并对其进行了麻疹多肽特异性检测。三个克隆与融合（F）蛋白发生特异性反应，一个识别血凝素（HA）。两个与完整病毒反应，但不与任何纯化蛋白反应。五个细胞系对多种麻疹多肽产生增殖反应。向两个多特异性细胞系中添加抗HA或抗F单克隆抗体，每个都导致细胞系对完整病毒的增殖反应部分受到抑制。三个F反应性克隆中的两个与HLA-DR4的一个亚组相关联识别抗原；第三个仅在自体抗原呈递细胞存在的情况下对F作出反应。在仅与完整病毒反应的两个克隆中，一个受限于DP3，另一个受限于自体细胞。HA特异性克隆受DP3限制。几个细胞系与单个HLA抗原相关联识别多种麻疹多肽。对单个麻疹多肽的识别并不与特定的遗传限制元件分离。
