Department of Food Hygiene, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Braz J Microbiol. 2013 Oct 30;44(2):393-9. doi: 10.1590/S1517-83822013000200008. eCollection 2013.
This study was conducted to determine the prevalence rate, enterotoxigenecity, and antimicrobial resistance of S. aureus isolated from dairy products in Iran. From September 2010 to July 2011, a total of 347 samples from various dairy products, traditional and commercial, were collected from randomly selected retail stores. Overall, 20 samples (5.8%) were found to be contaminated with S. aureus. The highest prevalence of S. aureus was found in traditional cheese (11.1%), followed by traditional ice-cream (5.9%), cream (5.6%), and butter (5.3%). The ability to synthesize classical staphylococcal enterotoxins (SEA-E) was determined in 7 of 20 (35%) isolates by using ELISA. SE type C was the most common enterotoxin found in the isolated S. aureus (42.9%), followed by SE type A (28.6%), SEA+SEC and SE type D (14.3%). Of the 20 isolates, 16 (80.0%) were positive for one or more entrotoxin genes and 8 different genotypes were observed. Susceptibilities of the isolates were determined for 14 antimicrobial drugs using the disk diffusion assay. Most of the isolates (95.0%) were resistant to one or more two antimicrobial agent and 45.0% of the isolates were resistant to three or more of drugs. Resistance to ampicillin was the most common finding (55.0%), followed by tetracycline (40.0%) and penicillin G (30.0%). The results of this study showed the wide spread of enterotoxigenic and multidrug-resistant S. aureus strains in traditional dairy products in Iran and highlighted their public health hazards.
本研究旨在确定伊朗乳制品中分离的金黄色葡萄球菌的流行率、肠毒性和抗菌药物耐药性。2010 年 9 月至 2011 年 7 月，从随机选择的零售店采集了 347 份来自各种乳制品、传统乳制品和商业乳制品的样本。总的来说，20 份样本（5.8%）被发现受到金黄色葡萄球菌污染。金黄色葡萄球菌的最高检出率出现在传统奶酪（11.1%），其次是传统冰淇淋（5.9%）、奶油（5.6%）和黄油（5.3%）。通过 ELISA 法确定了 20 个分离株中的 7 个（35%）能够合成经典的葡萄球菌肠毒素（SEA-E）。分离出的金黄色葡萄球菌中最常见的肠毒素是 SE 型 C（42.9%），其次是 SE 型 A（28.6%）、SEA+SEC 和 SE 型 D（14.3%）。在 20 个分离株中，16 个（80.0%）对一种或多种肠毒素基因呈阳性，观察到 8 种不同的基因型。使用纸片扩散法测定了 14 种抗菌药物对分离株的敏感性。大多数分离株（95.0%）对一种或多种两种抗菌药物耐药，45.0%的分离株对三种或更多药物耐药。对氨苄青霉素的耐药性最常见（55.0%），其次是四环素（40.0%）和青霉素 G（30.0%）。本研究结果表明，肠毒性和多药耐药性金黄色葡萄球菌在伊朗传统乳制品中广泛传播，突出了其对公共健康的危害。
