伊朗当地市场糖果中食源性病原体和抗生素抗性基因的流行情况
Prevalence of Foodborne Bacterial Pathogens and Antibiotic Resistance Genes in Sweets from Local Markets in Iran.
作者信息
Pakbin Babak, Amani Zahra, Rahimi Zahra, Najafi Somayeh, Familsatarian Behnaz, Khakpoor Alireza, Brück Wolfram Manuel, Brück Thomas B
机构信息
Werner Siemens Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich (TUM), Lichtenberg Str. 4, 85748 Garching bei München, Germany.
Institute for Life Technologies, University of Applied Sciences Western Switzerland Valais-Wallis, 1950 Sion 2, Switzerland.
出版信息
Foods. 2023 Oct 2;12(19):3645. doi: 10.3390/foods12193645.
BACKGROUND
This study aimed to investigate the prevalences of some important antibiotic-resistance genes (ARGs) and foodborne bacterial pathogens in sweet samples collected from local markets in Iran.
METHODS
Forty sweet samples were collected. Foodborne pathogens and ARGs were detected in the sweet samples by conventional and multiplex PCR assays using species-specific primers.
RESULTS
, , spp., , and were detected and identified in 47.5%, 20%, 45%, 5%, and 30% of the sweet samples, respectively. We found and spp. were the most prevalent bacterial pathogens. was found to be the most frequent pathogenic bacteria profiled in these samples. We also found a significant correlation between the presence of and . We detected the resistance gene in 97.5% of the sweet samples; however, was detected in only one sample (2.5%).
CONCLUSIONS
Regarding these results, we suggest preventive strategies such as implementing automation of food processing; monitoring the personal hygiene and health of food handlers, and testing regularly for antibiotic resistance in raw materials and products.
背景
本研究旨在调查从伊朗当地市场采集的甜食样本中一些重要抗生素抗性基因（ARGs）和食源性病原体的流行情况。
方法
采集了40份甜食样本。使用物种特异性引物，通过常规和多重PCR检测法在甜食样本中检测食源性病原体和ARGs。
结果
在47.5%、20%、45%、5%和30%的甜食样本中分别检测到并鉴定出 、 、 属、 和 。我们发现 属和 属是最普遍的细菌病原体。 被发现是这些样本中最常见的病原菌。我们还发现 和 的存在之间存在显著相关性。在97.5%的甜食样本中检测到 抗性基因；然而，仅在一个样本（2.5%）中检测到 。
结论
基于这些结果，我们建议采取预防策略，如实现食品加工自动化；监测食品处理人员的个人卫生和健康状况，并定期检测原材料和产品中的抗生素抗性。
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