A Saberi Eshagh, Farhadmollashahi Narges, Ghotbi Faroogh, Karkeabadi Hamed, Havaei Roholla
Assistant Professor, Department of Endodontics, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
Assistant Professor, Department of Endodontics, Faculty of Dentistry, Urmia University of Medical Sciences, Urmia, Iran.
J Dent Res Dent Clin Dent Prospects. 2016 Spring;10(2):75-80. doi: 10.15171/joddd.2016.012. Epub 2016 Jun 15.
Background. This in vitro study compared the effects of mineral trioxide aggregate (MTA), calcium enriched mixture(CEM) cement, Biodentine (BD) and octacalcium phosphate (OCP) on the viability of human gingival fibroblasts (HGFs). Methods. After completion of the setting time of the materials under study, fibroblasts were placed in 24-well insert platesand 1 mg of each material was added to the respective wells. The plates were then incubated at 37°C. The inserts were removedat 24, 48 and 168 hours and 2,5-diphenyltetrazolium bromide was added to assess cytotoxicity via the MTT colorimetricassay. Data were analyzed at different time intervals using repeated-measures ANOVA, followed by the Bonferronitest at three levels of significance of P < 0.05, P < 0.01 and P < 0.001. Results. Cytotoxicity of the materials under study was not significantly different at 24 and 48 hours compared to the controlgroup. However, at 168 hours, a significant difference was noted between MTA (P < 0.05) and Biodentine (P < 0.01)and the control group. Conclusion. Cytotoxicity of MTA, CEM, Biodentine and OCP against HGFs was similar to that of the control group at 24and 48 hours. Over time, MTA and Biodentine exhibited less cytotoxicity than other materials.
背景。本体外研究比较了三氧化矿物凝聚体（MTA）、富钙混合物（CEM）水泥、生物陶瓷（BD）和磷酸八钙（OCP）对人牙龈成纤维细胞（HGFs）活力的影响。方法。在研究材料凝固时间结束后，将成纤维细胞置于24孔插入板中，并向各孔中加入1mg每种材料。然后将平板在37°C下孵育。在24、48和168小时取出插入物，并加入2,5-二苯基四氮唑溴盐通过MTT比色法评估细胞毒性。使用重复测量方差分析在不同时间间隔分析数据，随后进行Bonferroni检验，显著性水平为P < 0.05、P < 0.01和P < 0.001。结果。与对照组相比，在24和48小时时，所研究材料的细胞毒性无显著差异。然而，在168小时时，MTA（P < 0.05）和生物陶瓷（P < 0.01）与对照组之间存在显著差异。结论。在24和48小时时，MTA、CEM、生物陶瓷和OCP对HGFs的细胞毒性与对照组相似。随着时间的推移，MTA和生物陶瓷比其他材料表现出更低的细胞毒性。
