Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Carbohydr Polym. 2014 Jan 30;101:188-95. doi: 10.1016/j.carbpol.2013.09.018. Epub 2013 Sep 16.
A water-soluble heteropolysaccharide (PS) was isolated from extracellular polymeric substances (EPS) produced by a novel metal tolerant bacterium, Acinetobacter junii BB1A. Sugar analysis showed that the PS was composed of mannose, galactose and arabinose in a molar ratio of nearly 3:1:1. Structural characterization of the PS was carried out using methylation analysis, periodate oxidation, Smith degradation and 1D/2D NMR experiments. Methylation analysis revealed that the PS was consisted of 2,4-linked-mannopyranosyl, 3,4-linked-mannopyranosyl, 2-linked-galactopyranosyl, terminal mannopyranosyl and arabinopyranosyl residues in a relative proportion of nearly 1:1:1:1:1. Smith degradation of the PS showed the presence of hydrated glyceraldehyde containing disaccharide unit consisting of α-d-Manp-(1→and→4)-α-d-Manp-(1→residues where the later was directly attached to a hydrated glyceraldehyde moiety. This polysaccharide showed significant in vitro splenocyte, thymocyte, and macrophage activations with optimum dose of 100 μg/mL for macrophage and 25 μg/mL both for the splenocyte and thymocyte.
从一株新型耐金属细菌——不动杆菌属 BB1A 的胞外聚合物（EPS）中分离得到一种水溶性杂多糖（PS）。糖分析表明，PS 由甘露糖、半乳糖和阿拉伯糖组成，摩尔比接近 3:1:1。通过甲基化分析、高碘酸氧化、Smith 降解和 1D/2D NMR 实验对 PS 的结构进行了表征。甲基化分析表明，PS 由 2,4-连接的甘露吡喃糖基、3,4-连接的甘露吡喃糖基、2-连接的半乳糖吡喃糖基、末端甘露吡喃糖基和阿拉伯吡喃糖基残基组成，相对比例接近 1:1:1:1:1。PS 的 Smith 降解表明存在含有二糖单元的水合甘油醛，该二糖单元由α-d-Manp-(1→和→4)-α-d-Manp-(1→残基组成，后者直接连接到水合甘油醛部分。该多糖在体外对脾细胞、胸腺细胞和巨噬细胞具有显著的激活作用，最佳剂量为巨噬细胞 100 μg/mL，脾细胞和胸腺细胞 25 μg/mL。
