School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Molecules. 2019 Feb 19;24(4):749. doi: 10.3390/molecules24040749.
Saffron, a kind of rare medicinal herb with antioxidant, antitumor, and anti-inflammatory activities, is the dry stigma of L. A new water-soluble endophytic exopolysaccharide (EPS-2) was isolated from saffron by anion exchange chromatography and gel filtration. The chemical structure was characterized by FT-IR, GC-MS, and 1D and 2D-NMR spectra, indicating that EPS-2 has a main backbone of (1→2)-linked α-d-Manp, (1→2, 4)-linked α-d-Manp, (1→4)-linked α-d-Xylp, (1→2, 3, 5)-linked β-d-Araf, (1→6)- linked α-d-Glcp with α-d-Glcp-(1→ and α-d-Galp-(1→ as sidegroups. Furthermore, EPS-2 significantly attenuated gentamicin-induced cell damage in cultured HEI-OC1 cells and increased cell survival in zebrafish model. The results suggested that EPS-2 could protect cochlear hair cells from ototoxicity exposure. This study could provide new insights for studies on the pharmacological mechanisms of endophytic exopolysaccharides from saffron as otoprotective agents.
藏红花，一种具有抗氧化、抗肿瘤和抗炎活性的稀有药用植物，是 L. 的干燥柱头。一种新的水溶性内生胞外多糖 (EPS-2) 已通过阴离子交换色谱和凝胶过滤从藏红花中分离出来。通过傅里叶变换红外光谱 (FT-IR)、气相色谱-质谱联用 (GC-MS) 和 1D 和 2D-NMR 光谱对其化学结构进行了表征，表明 EPS-2 具有由 (1→2)-连接的α-d-Manp、(1→2,4)-连接的α-d-Manp、(1→4)-连接的α-d-Xylp、(1→2,3,5)-连接的β-d-Araf、(1→6)-连接的α-d-Glcp 组成的主链，以及α-d-Glcp-(1→和α-d-Galp-(1→作为侧基。此外，EPS-2 显著减轻了庆大霉素诱导的培养 HEI-OC1 细胞损伤，并增加了斑马鱼模型中的细胞存活率。结果表明，EPS-2 可保护耳蜗毛细胞免受耳毒性暴露。本研究可为藏红花内生胞外多糖作为耳保护剂的药理机制研究提供新的见解。
