Zhang Jiaozhen, Liu Na, Sun Chao, Sun Deqing, Wang Yongjie
Department of Natural Products Chemistry, Key Lab of Chemical Biology of Ministry of Education, Shandong University Jinan Shandong 250012 PR China.
Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University Jinan Shandong 250012 PR China
RSC Adv. 2019 Jun 7;9(31):17988-17994. doi: 10.1039/c9ra03023a. eCollection 2019 Jun 4.
The aim of this study was to examine the effect of polysaccharides from (PSP) on RAW 264.7 cells together with the underlying signaling pathways. Water-soluble polysaccharides were extracted from and the immunological activity/mechanism was explored in depth in RAW 264.7 cells. Our results demonstrated that PSP induced dendritic-like morphological changes in RAW 264.7 cells, and increased the production of nitric oxide, TNF-α and IL-6 in a dose-dependent manner. Mechanistic studies revealed that PSP promoted the accumulation of TNF-α and IL-6 mRNA and caused IκB-α degradation and NF-κB p65 translocation into the nucleus at both the transcriptional and translational levels. The expression of iNOS, COX-2, NF-κB and phosphorylated p38 MAPK was upregulated in PSP-treated RAW 264.7 cells. Water-soluble polysaccharides possess immunological activity and the immunostimulatory mechanism may be attributed to the NF-κB and p38 MAPK pathways similar to the mechanism of lipopolysaccharides.
本研究的目的是研究[具体名称]多糖（PSP）对RAW 264.7细胞的作用及其潜在的信号通路。从[具体名称]中提取水溶性多糖，并在RAW 264.7细胞中深入探究其免疫活性/机制。我们的结果表明，PSP诱导RAW 264.7细胞出现树突状形态变化，并以剂量依赖的方式增加一氧化氮、TNF-α和IL-6的产生。机制研究表明，PSP在转录和翻译水平上促进TNF-α和IL-6 mRNA的积累，导致IκB-α降解以及NF-κB p65易位至细胞核。在经PSP处理的RAW 264.7细胞中，iNOS、COX-2、NF-κB和磷酸化p38 MAPK的表达上调。水溶性多糖具有免疫活性，其免疫刺激机制可能与脂多糖的机制类似，归因于NF-κB和p38 MAPK信号通路。
