Guangxi Medical University, Nanning, Guangxi 530021, China.
Carbohydr Polym. 2014 Jan 30;101:533-43. doi: 10.1016/j.carbpol.2013.09.037. Epub 2013 Sep 23.
A polysaccharide (PMP) was isolated from Millettia pulchra and purified by DEAE-cellulose and Sephadex G-75 chromatography. The results showed that PMP was composed of d-glucose and d-arabinose in a molar ratio of 90.79% and 9.21%, with an average molecular weight of about 14,301 Da. Furthermore, the effect of PMP on cognitive impairment induced by d-galactose in mice was evaluated. Treatment with PMP significantly reversed d-galactose-induced learning and memory impairments, as measured by behavioral tests. One of the potential mechanisms of this action was to reduce oxidative stress and suppress inflammatory responses. Furthermore, our results also showed that PMP markedly reduced the content and deposition of β-amyloid peptide, improved the dysfunction of synaptic plasticity, increased the levels of acetylcholine, but decreased cholinesterase activity. These results suggest that PMP exerts an effective protection against d-galactose-induced cognitive impairment, and PMP may be a major bioactive ingredient in M. pulchra.
从美丽崖豆藤中分离得到一种多糖（PMP），并通过 DEAE-纤维素和 Sephadex G-75 层析进行纯化。结果表明，PMP 由摩尔比为 90.79%和 9.21%的 d-葡萄糖和 d-阿拉伯糖组成，平均分子量约为 14301 Da。此外，还评价了 PMP 对 d-半乳糖诱导的小鼠认知障碍的影响。行为测试结果表明，PMP 处理可显著逆转 d-半乳糖诱导的学习和记忆障碍。这种作用的潜在机制之一是减轻氧化应激和抑制炎症反应。此外，我们的结果还表明，PMP 可显著降低β-淀粉样肽的含量和沉积，改善突触可塑性的功能障碍，增加乙酰胆碱水平，但降低胆碱酯酶活性。这些结果表明 PMP 对 d-半乳糖诱导的认知障碍具有有效的保护作用，PMP 可能是美丽崖豆藤中的主要生物活性成分。
