Hydrosciences Montpellier UMR 5569, Department of Environmental Sciences and Public Health, University of Montpellier, I, 34000, Montpellier, France,
Anal Bioanal Chem. 2014 Jan;406(2):601-10. doi: 10.1007/s00216-013-7477-x. Epub 2013 Dec 4.
Coastal areas are subject to growing pressures and impacts because of the increase in human activities. Lipophilic organic contaminants, such as polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs), have been monitored for decades within monitoring programs. However, until now, little information on the detection of so-called "emerging contaminants" such as hydrophilic organic compounds in the marine environment and no data on its metabolites or transformation products in marine organisms is available. In this report, a sensitive analytical methodology for identification and confirmation of venlafaxine (VEN) residues and five of its main metabolites in the marine mussels Mytilus galloprovincialis was validated. The sample preparation procedure was based on the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) approach. An analytical method was developed to quantify these compounds at trace levels by liquid chromatography coupled to high-resolution mass spectrometry. The method was then applied to marine mussels collected from the Mediterranean Sea in southeastern France. Residues of the antidepressant VEN were occasionally detected at ng/g dw level. In addition, the approach allowed us to identify several transformation products in the analyzed samples. N-desmethylvenlafaxine (NDV) was the most frequently detected metabolite followed by N,O-di-desmethylvenlafaxine (NODDV).
由于人类活动的增加，沿海地区面临着越来越大的压力和影响。在监测计划中，已经对多环芳烃（PAHs）或多氯联苯（PCBs）等亲脂性有机污染物进行了数十年的监测。然而，直到现在，关于海洋环境中所谓的“新兴污染物”（如亲水性有机化合物）的检测信息仍然很少，海洋生物中也没有其代谢物或转化产物的数据。在本报告中，验证了一种用于鉴定和确证海洋贻贝贻贝（Mytilus galloprovincialis）中文拉法辛（VEN）残留及其五种主要代谢物的灵敏分析方法。样品制备程序基于快速、简便、廉价、有效、坚固和安全（QuEChERS）方法。开发了一种分析方法，通过液相色谱-高分辨率质谱联用技术对这些化合物进行痕量水平的定量。然后将该方法应用于从法国东南部地中海采集的贻贝。在 ng/g dw 水平偶尔检测到抗抑郁药 VEN 的残留。此外，该方法还允许我们在分析的样品中鉴定出几种转化产物。检测到的最常见代谢物是去甲基文拉法辛（NDV），其次是 N,O-二去甲基文拉法辛（NODDV）。
