Rubinstein M, Orchansky P
CRC Crit Rev Biochem. 1986;21(3):249-75. doi: 10.3109/10409238609113613.
Early studies on the mode of action of interferons have indicated that a receptor system on the cell surface is involved in its action. The first direct evidence to a high-affinity binding site was found only after pure interferon was available. Two different receptors, one specific for interferons-alpha and beta, and the other for interferon-gamma were recognized. A correlation between affinity to the receptor and specific activity was established. Cross-linked complexes of labeled interferons with their receptors were visualized on gel electrophoresis and even partially purified. Internalization of interferons after binding to the receptor was reported. The role of gangliosides as helpers of interferon binding was recently investigated. Fragments of interferons which still retained binding capacity were described and helped in elucidating the binding site on the interferon molecule.
早期关于干扰素作用方式的研究表明，细胞表面的受体系统参与其作用。只有在获得纯干扰素后，才发现了高亲和力结合位点的首个直接证据。人们识别出两种不同的受体，一种对α干扰素和β干扰素具有特异性，另一种对γ干扰素具有特异性。建立了与受体的亲和力和比活性之间的相关性。标记干扰素与其受体的交联复合物在凝胶电泳上可见，甚至经过了部分纯化。有报道称干扰素与受体结合后会发生内化。最近研究了神经节苷脂作为干扰素结合辅助因子的作用。描述了仍保留结合能力的干扰素片段，这有助于阐明干扰素分子上的结合位点。
