Suga T, Maeda Y Y, Uchida H, Rokutanda M, Chihara G
Int J Immunopharmacol. 1986;8(7):691-9. doi: 10.1016/0192-0561(86)90004-4.
A new bioactive factor capable of stimulating the production of acute-phase transport proteins, haptoglobin, hemopexin and ceruloplasmin, was found in mouse serum soon after the administration of lentinan, an immunomodulatory polysaccharide. This factor (APPIF) was produced by macrophages, and may regulate the production of acute-phase transport proteins in hepatocytes. The mice given the serum obtained from donor mice 2-6 h after an injection of 10 mg/kg of lentinan showed a marked increase of the acute-phase transport proteins in their serum 4 days after the serum injection. Pretreatment with the antimacrophage agents, carrageenan and mouse Ia antiserum, before the lentinan treatment to donor mice inhibited the production of acute-phase transport proteins in the recipient mice. Thymus or T-cells had no role in the production of APPIF. As the activity of APPIF disappeared after pronase treatment of the serum, APPIF seems to be a peptide compound. Appearance of APPIF is considered to be one of the earliest manifestations of the mode of action of lentinan in addition to its augmented production of vascular dilatation and hemorrhage inducing factor and interleukin-1. The correlation between these inflammatory and immune responses in earlier stages of the host defence mechanisms is also discussed.
在给予香菇多糖（一种免疫调节多糖）后不久，在小鼠血清中发现了一种新的生物活性因子，它能够刺激急性期转运蛋白、触珠蛋白、血红素结合蛋白和铜蓝蛋白的产生。这种因子（APPIF）由巨噬细胞产生，可能调节肝细胞中急性期转运蛋白的产生。给小鼠注射10mg/kg香菇多糖后2 - 6小时，从供体小鼠获得的血清注射到受体小鼠体内，4天后受体小鼠血清中的急性期转运蛋白显著增加。在给供体小鼠注射香菇多糖之前，用抗巨噬细胞剂角叉菜胶和小鼠Ia抗血清进行预处理，可抑制受体小鼠中急性期转运蛋白的产生。胸腺或T细胞在APPIF的产生中不起作用。由于血清经链霉蛋白酶处理后APPIF的活性消失，APPIF似乎是一种肽化合物。APPIF的出现被认为是香菇多糖作用方式的最早表现之一，此外还有其血管扩张和出血诱导因子以及白细胞介素 - 1产生的增加。还讨论了宿主防御机制早期这些炎症和免疫反应之间的相关性。
