Rowett Research Institute, Bucksburn, AB2 9SB, Aberdeen, U.K..
Planta. 1979 Dec;147(2):103-10. doi: 10.1007/BF00389508.
A method is described for the sub-cellular ractionation of cotyledons from non-germinated Phaseolus vulgaris seeds in non-aqueous density gradients of potassium iodide in glycerol. The major organelles (protein bodies, cell walls and starch grains) are well resolved as adjudged by morphology and protein subunit patterns of gradient zones and are remarkably stable if water is rigorously excluded. The flexibility of the system is exploited in establishing the cytoplasmic location of the oligosaccharide fraction and in the large-scale preparative isolation of the sub-cellular components. The composition and morphology of the sub-cellular components are described and discussed.
描述了一种从小豆（ Phaseolus vulgaris ）未萌发种子的子叶中分离细胞器的方法，该方法使用碘化钾-甘油非水密度梯度。通过梯度区的形态和蛋白质亚基模式判断，主要细胞器（蛋白体、细胞壁和淀粉粒）得到了很好的分离，如果严格排除水分，这些细胞器非常稳定。该系统的灵活性在确定寡糖部分的细胞质位置和大规模制备分离亚细胞成分方面得到了利用。描述和讨论了亚细胞成分的组成和形态。
