姜黄素纳米颗粒与游离姜黄素对乳腺癌人端粒酶逆转录酶基因表达抑制作用的比较
Comparison of inhibitory effect of curcumin nanoparticles and free curcumin in human telomerase reverse transcriptase gene expression in breast cancer.
作者信息
Kazemi-Lomedasht Fatemeh, Rami Abbas, Zarghami Nosratolla
机构信息
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Pasteur Institute of Iran, Tehran, Iran.
出版信息
Adv Pharm Bull. 2013;3(1):127-30. doi: 10.5681/apb.2013.021. Epub 2013 Feb 7.
Abstract
PURPOSE
Telomerase is expressed in most cancers, including breast cancer. Curcumin, a polyphenolic compound that obtained from the herb of Curcuma longa, has many anticancer effects. But, its effect is low due to poor water solubility. In order to improve its solubility and drug delivery, we have utilized a β-cyclodextrin-curcumin inclusion complex.
METHODS
To evaluate cytotoxic effects of cyclodextrin-curcumin and free curcumin, MTT assay was done. Cells were treated with equal concentration of cyclodextrin-curcumin and free curcumin. Telomerase gene expression level in two groups was compared by Real-time PCR.
RESULTS
MTT assay demonstrated that β-cyclodextrin-curcumin enhanced curcumin delivery in T47D breast cancer cells. The level of telomerase gene expression in cells treated with cyclodextrin-curcumin was lower than that of cells treated with free curcumin (P=0.001).
CONCLUSION
RESULTS are suggesting that cyclodextrin-curcumin complex can be more effective than free curcumin in inhibition of telomerase expression.
摘要
目的
端粒酶在包括乳腺癌在内的大多数癌症中均有表达。姜黄素是一种从姜黄属植物中提取的多酚类化合物，具有多种抗癌作用。但其水溶性差，导致其效果不佳。为了提高其溶解度和药物递送能力，我们使用了β-环糊精-姜黄素包合物。
方法
为评估环糊精-姜黄素和游离姜黄素的细胞毒性作用，进行了MTT试验。用等浓度的环糊精-姜黄素和游离姜黄素处理细胞。通过实时PCR比较两组中端粒酶基因的表达水平。
结果
MTT试验表明，β-环糊精-姜黄素增强了姜黄素在T47D乳腺癌细胞中的递送。用环糊精-姜黄素处理的细胞中端粒酶基因表达水平低于用游离姜黄素处理的细胞（P = 0.001）。
结论
结果表明，环糊精-姜黄素复合物在抑制端粒酶表达方面可能比游离姜黄素更有效。
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本文引用的文献
1
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Biochem Pharmacol. 2010 Oct 1;80(7):1021-32. doi: 10.1016/j.bcp.2010.06.022. Epub 2010 Jun 23.
2
beta-Cyclodextrin-curcumin self-assembly enhances curcumin delivery in prostate cancer cells.β-环糊精-姜黄素自组装增强姜黄素在前列腺癌细胞中的递送。
Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):113-25. doi: 10.1016/j.colsurfb.2010.03.039. Epub 2010 Apr 3.
3
Female breast cancer mortality clusters within racial groups in the United States.美国不同种族群体的女性乳腺癌死亡率存在聚集现象。
Health Place. 2010 Mar;16(2):209-18. doi: 10.1016/j.healthplace.2009.09.012. Epub 2009 Oct 4.
4
Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins.姜黄素通过与多种细胞信号蛋白相互作用，抑制不同癌症的增殖、侵袭、血管生成和转移。
Cancer Lett. 2008 Oct 8;269(2):199-225. doi: 10.1016/j.canlet.2008.03.009. Epub 2008 May 13.
5
Pharmacological intervention strategies for affecting telomerase activity: future prospects to treat cancer and degenerative disease.影响端粒酶活性的药理学干预策略：治疗癌症和退行性疾病的未来前景
Biochimie. 2008 Jan;90(1):156-72. doi: 10.1016/j.biochi.2007.09.002. Epub 2007 Sep 11.
6
Curcumin: the Indian solid gold.姜黄素：印度的固体黄金。
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7
Cyclodextrins in drug delivery: an updated review.环糊精在药物递送中的应用：最新综述
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8
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Eur J Cancer. 2005 Sep;41(13):1955-68. doi: 10.1016/j.ejca.2005.05.009.
9
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Breast Cancer Res Treat. 2003 Feb;77(3):277-84. doi: 10.1023/a:1021849217054.
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