光果独活种子乙醇提取物对癌细胞活力的细胞毒性作用。
Cytotoxic effects of alcoholic extract of dorema glabrum seed on cancerous cells viability.
作者信息
Bannazadeh Amirkhiz Maryam, Rashtchizadeh Nadereh, Nazemieh Hosein, Abdolalizadeh Jalal, Mohammadnejad Leila, Baradaran Behzad
机构信息
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; student of Tabriz International University of Medical Sciences (Aras), Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Adv Pharm Bull. 2013;3(2):403-8. doi: 10.5681/apb.2013.064. Epub 2013 Aug 20.
PURPOSE
In the present study cytotoxic effects of the alcoholic extract of Dorema Glabrum seed on viability of WEHI-164 cells, mouse Fibrosarcoma cell line and L929 normal cells were compared with the cytotoxic effects of Taxol (anticancer and apoptosis inducer drug).
METHODS
To find out the plant extract cytotoxic effects, MTT test and DNA fragmentation assay, the biochemical hallmark of apoptosis were performed on cultured and treated cells.
RESULTS
According to the findings the alcoholic extract of Dorema Glabrum seed can alter cells morphology and because of chromatin condensation and other changes they shrink and take a spherical shape, and lose their attachment too. So the plant extract inhibits cell growth albeit in a time and dose dependent manner and results in degradation of chromosomal DNA.
CONCLUSION
Our data well established the anti-proliferative effect of methanolic extract of Dorema Glabrum seed and clearly showed that the plant extract can induce apoptosis and not necrosis in vitro, but the mechanism of its activities remained unknown. These results demonstrated that Dorema Glabrum seed might be a novel and attractive therapeutic candidate for tumor treatment in clinical practices.
目的
在本研究中，将多蕾麻籽酒精提取物对WEHI-164细胞、小鼠纤维肉瘤细胞系和L929正常细胞活力的细胞毒性作用与紫杉醇（抗癌和凋亡诱导药物）的细胞毒性作用进行比较。
方法
为了确定植物提取物的细胞毒性作用，对培养和处理后的细胞进行了MTT试验和DNA片段化分析，这是凋亡的生化标志。
结果
根据研究结果，多蕾麻籽酒精提取物可改变细胞形态，由于染色质浓缩和其他变化，细胞会收缩并呈球形，还会失去附着能力。因此，植物提取物能抑制细胞生长，且呈时间和剂量依赖性，并导致染色体DNA降解。
结论
我们的数据充分证实了多蕾麻籽甲醇提取物的抗增殖作用，并清楚地表明该植物提取物在体外可诱导凋亡而非坏死，但其活性机制仍不清楚。这些结果表明，多蕾麻籽可能是临床实践中一种新型且有吸引力的肿瘤治疗候选药物。
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