Alharbi Samir Abdulkarim
Department of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia.
J Pharm Bioallied Sci. 2024 Dec;16(Suppl 4):S3354-S3357. doi: 10.4103/jpbs.jpbs_631_24. Epub 2024 Sep 5.
(Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells.
The current study determined the antioxidant activity and the anticancer potential of methanolic extract of against gastric carcinoma.
LC-MS analysis was used to determine the different phytoconstituents predicted to be present in the extract. The effect of the leaf extract against human gastric carcinoma (AGS) cell lines was evaluated using MTT assay and cellular apoptosis techniques. A ferric-reducing antioxidant power assay was used to assess the antioxidant effect.
The LC-MS analysis revealed 24 phytoconstituents and extract was cytotoxic on AGS cells with an IC value of 125.1 μg/ml. Incubation of AGS cells with the extract decreased the percentage of live cells with a significant increase in late apoptotic phase and necrotic phase in the cellular apoptosis assay. The extract showed a potent antioxidant effect. The results show that extract possesses cytotoxic and antioxidant actions.
鼠李科植物在中东国家被用于治疗各种疾病。早期报告显示，它对口腔癌细胞具有细胞毒性。
本研究测定了[鼠李科植物名]甲醇提取物对胃癌的抗氧化活性和抗癌潜力。
采用液相色谱 - 质谱联用（LC - MS）分析来确定提取物中预计存在的不同植物成分。使用MTT法和细胞凋亡技术评估[鼠李科植物名]叶提取物对人胃癌（AGS）细胞系的作用。采用铁还原抗氧化能力测定法评估抗氧化效果。
LC - MS分析揭示了24种植物成分，[鼠李科植物名]提取物对AGS细胞具有细胞毒性，IC值为125.1μg/ml。在细胞凋亡试验中，用提取物孵育AGS细胞可降低活细胞百分比，同时晚期凋亡阶段和坏死阶段显著增加。[鼠李科植物名]提取物显示出强大的抗氧化作用。结果表明，[鼠李科植物名]提取物具有细胞毒性和抗氧化作用。
