Department of Chemical and Biomolecular Engineering, National University of Singapore , 4 Engineering Drive 4, Singapore 117585, Singapore.
Nano Lett. 2014 Jan 8;14(1):83-8. doi: 10.1021/nl4032549. Epub 2013 Dec 18.
Nanoparticles can have profound effects on cell biology. Here, we show that after TiO2, SiO2, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.
纳米颗粒会对细胞生物学产生深远的影响。在这里，我们表明，经过 TiO2、SiO2 和羟基磷灰石纳米颗粒处理后，TR146 上皮细胞片的迁移速度变慢。暴露于纳米颗粒后的细胞表现出更高的细胞收缩性，伤口愈合能力显著受损，但没有明显的细胞毒性。我们表明，其机制是通过纳米颗粒介导的细胞内微管组装的大量破坏，从而引发正向反馈，促进更强的基底附着，从而导致细胞迁移受限。
