Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
Gene. 2014 Feb 10;535(2):150-5. doi: 10.1016/j.gene.2013.11.045. Epub 2013 Dec 6.
We determined a draft genome sequence for Moorella thermoacetica strain Y72, a syngas-assimilating bacterium with high transformation efficiency. This strain was confirmed to be M. thermoacetica because its overall genome sequence characteristics were similar to those of M. thermoacetica strain ATCC39073. Y72 was confirmed to carry all the genes encoding the enzymes in the reductive acetyl-CoA pathway, with very high similarities to those of ATCC39073. In addition, it was confirmed to assimilate carbon dioxide using this pathway. However, although both Y72 and ATCC39073 carried common genes encoding several enzymes related to the reductive tricarboxylic acid (TCA) cycle, their gene sets were different. Our results suggested that the reason for higher transformation efficiency in Y72 than that in ATCC39073, a reference strain of M. thermoacetica, may be that Y72 possesses only 2 sets of genes considered to be involved in a restriction-modification system, which was half of those found in ATCC39073.
我们确定了一株具有高效转化能力的合成气同化细菌 Moorella thermoacetica 菌株 Y72 的草图基因组序列。该菌株被确认为 M. thermoacetica，因为其全基因组序列特征与 ATCC39073 菌株相似。Y72 被证实携带还原乙酰辅酶 A 途径中所有编码酶的基因，与 ATCC39073 非常相似。此外，它被证实可以通过该途径同化二氧化碳。然而，尽管 Y72 和 ATCC39073 都携带编码与还原三羧酸 (TCA) 循环相关的几种酶的常见基因，但它们的基因集不同。我们的结果表明，Y72 的转化效率高于 M. thermoacetica 的参考菌株 ATCC39073 的原因可能是，Y72 只拥有 2 组被认为与限制-修饰系统有关的基因，而 ATCC39073 则有 4 组。
