Departments of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States.
Departments of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States; Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States.
FEBS Lett. 2014 Jan 21;588(2):253-60. doi: 10.1016/j.febslet.2013.11.035. Epub 2013 Dec 6.
Mammalian protein production platforms have had a profound impact in many areas of basic and applied research, and an increasing number of blockbuster drugs are recombinant mammalian proteins. With global sales of these drugs exceeding US$120 billion per year, both industry and academic research groups continue to develop cost effective methods for producing mammalian proteins to support pre-clinical and clinical evaluations of potential therapeutics. While a wide range of platforms have been successfully exploited for laboratory use, the bulk of recent biologics have been produced in mammalian cell lines due to the requirement for post translational modification and the biosynthetic complexity of the target proteins. In this review we highlight the range of mammalian expression platforms available for recombinant protein production, as well as advances in technologies for the rapid and efficient selection of highly productive clones.
哺乳动物蛋白生产平台在基础和应用研究的许多领域产生了深远的影响，越来越多的重磅炸弹药物是重组哺乳动物蛋白。这些药物的年销售额超过 1200 亿美元，因此工业界和学术研究小组继续开发具有成本效益的生产哺乳动物蛋白的方法，以支持潜在治疗药物的临床前和临床评估。虽然已经成功地利用了广泛的平台进行实验室使用，但由于需要翻译后修饰和目标蛋白的生物合成复杂性，最近的生物制剂大部分是在哺乳动物细胞系中生产的。在这篇综述中，我们强调了可用于重组蛋白生产的各种哺乳动物表达平台，以及在快速高效选择高产克隆方面的技术进展。
