Zeng Guofang, Zhong Fang, Li Jin, Luo Shaojun, Zhang Peihua
Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College.
Biosci Biotechnol Biochem. 2013;77(12):2389-96. doi: 10.1271/bbb.130502. Epub 2013 Dec 7.
Hypertrophic scar (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of extracellular matrix. Here, to investigate the regulative effects of resveratrol, a natural antioxidant compound, on fibroblasts from human skin HS tissue, a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the inhibitory effect of resveratrol on cells. Cellcycle progression and apoptosis were measured by flow cytometry and Hoechst 33258 staining respectively. The hydroxyproline content and mRNA expression levels of type I and III procollagen were measured separately by ELISA and reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that resveratrol significantly inhibited cell growth, arresting the cell cycle at the G1 phase and inducing apoptosis in the fibroblasts, decreasing hydroxyproline (or collagen) levels, and downregulating the expression levels of type I and III procollagen mRNA. Taken together, these data indicate that resveratrol-mediated reduction of collagen in fibroblasts is at least partially effected by causing inhibitory cell growth, cellcycle arrest, and apoptosis, and they suggest that resveratrol is a potential agent for HS treatment.
增生性瘢痕（HS）是一种真皮纤维增生性疾病，其特征在于细胞外基质过度沉积。在此，为了研究天然抗氧化化合物白藜芦醇对人皮肤HS组织成纤维细胞的调节作用，采用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）法评估白藜芦醇对细胞的抑制作用。分别通过流式细胞术和Hoechst 33258染色检测细胞周期进程和细胞凋亡。通过酶联免疫吸附测定（ELISA）和逆转录-聚合酶链反应（RT-PCR）分别测量羟脯氨酸含量以及I型和III型前胶原的mRNA表达水平。结果表明，白藜芦醇显著抑制细胞生长，使细胞周期停滞在G1期并诱导成纤维细胞凋亡，降低羟脯氨酸（或胶原蛋白）水平，并下调I型和III型前胶原mRNA的表达水平。综上所述，这些数据表明，白藜芦醇介导的成纤维细胞中胶原蛋白减少至少部分是通过抑制细胞生长、细胞周期停滞和凋亡实现的，并且表明白藜芦醇是一种治疗HS的潜在药物。
