Department of Horticultural Science and Landscape Architecture, University of Minnesota, St. Paul, MN, USA.
Theor Appl Genet. 1978 May;52(3):135-9. doi: 10.1007/BF00264747.
A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.
一种新的考马斯亮蓝 G-250 法已被应用于马铃薯块茎蛋白质改良育种计划的筛选方法中。该方法简单、快速、廉价，并成功地估算了具有不同氨基酸组成和块茎成熟度的广泛块茎基因型的总蛋白质含量。考马斯亮蓝法与改良的微量凯氏定氮法（马铃薯育种计划中使用的标准方法）之间的相关性为 0.93。不会干扰考马斯亮蓝法的游离氨基酸和其他干扰其他蛋白质测定方法的化合物。
