Min Young Ju, Park Myung Soo, Fong Jonathan J, Quan Ying, Jung Sungcheol, Lim Young Woon
School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
J Microbiol Biotechnol. 2014 Mar 28;24(3):324-33. doi: 10.4014/jmb.1310.10041.
The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.
黑松（Pinus thunbergii）广泛分布于韩国东海岸，在印度尼西亚和日本发生海啸后，其作为防护林的重要性凸显出来。在韩国的三个沿海地区——固城、蔚珍和釜山，对黑松的根内生菌多样性进行了调查。从根尖分离出真菌，并测量纯培养物在添加和不添加3%氯化钠的马铃薯葡萄糖琼脂（PDA）上的生长速率，以确定它们的耐盐性。总共259个分离株被分为136个形态型，其中通过内转录间隔区序列鉴定出58个物种。每个主要真菌门的代表都有：44个子囊菌门、8个接合菌门和6个担子菌门。18个物种表现出耐盐性，其中许多是青霉属和木霉属的物种。与内陆地区相比，海岸线栖息地具有更高的耐盐内生菌多样性。这项调查表明，生活在靠近海岸的黑松内生菌可能对盐分具有更高的抗性，并且可能与黑松存在特定关系。
