Owoyele Bamidele Victor, Oladejo Rasheed Olajiire, Ajomale Kayode, Ahmed Rasheedat Omotayo, Mustapha Abdulrasheed
University of Ilorin, Ilorin, Nigeria,
Metab Brain Dis. 2014 Mar;29(1):167-73. doi: 10.1007/s11011-013-9458-3. Epub 2013 Dec 10.
The use of honey for therapeutic purposes is on the increase and many studies have shown that honey has the ability to influence biological systems including pain transmission. Therefore, this study was designed to investigate the analgesic and anti-inflammatory effects of honey and the effects of concurrent administration of autonomic nervous system blocking drugs. Studies on analgesic activities was carried out using hotplate and formalin-induced paw licking models while the anti-inflammatory activity was by the carrageenan paw oedema method. Animals were distributed into six groups consisting of five animals each. They were administered saline, honey (600 mg/kg), indomethacin (5 mg/kg), autonomic blockers (3 μg/kg of tamsulosin, 20 mg/kg (intraperitoneally) of propranolol, 2 ml/kg of atropine or 10 mg/kg (intra muscularly) of hexamethonium) or honey (200 and 600 mg/kg) with one of the blockers. The results showed that honey reduced pain perception especially inflammatory pain and the administration of tamsulosin and propranolol spared the effect of honey. Hexamethonium also spared the effects of honey at the early and late phases of the test while atropine only inhibited the early phase of the test. However, atropine and hexamethonium spared the anti-inflammatory effects of honey but tamsulosin abolished the effects while propranolol only abolished the anti-inflammatory effects at the peak of the inflammation. The results suggest the involvement of autonomic receptors in the anti-nociceptive and anti-inflammatory effects of honey although the level of involvement depends on the different types of the receptors.
蜂蜜用于治疗目的的情况正在增加，许多研究表明蜂蜜有能力影响包括疼痛传递在内的生物系统。因此，本研究旨在调查蜂蜜的镇痛和抗炎作用以及自主神经系统阻断药物联合给药的效果。使用热板和福尔马林诱导的舔爪模型进行镇痛活性研究，而通过角叉菜胶致爪肿胀法研究抗炎活性。将动物分为六组，每组五只动物。它们分别接受生理盐水、蜂蜜（600毫克/千克）、吲哚美辛（5毫克/千克）、自主神经阻滞剂（坦索罗辛3微克/千克、普萘洛尔20毫克/千克（腹腔注射）、阿托品2毫升/千克或六甲铵10毫克/千克（肌肉注射））或蜂蜜（200和600毫克/千克）与一种阻滞剂联合给药。结果表明，蜂蜜可降低疼痛感知，尤其是炎性疼痛，坦索罗辛和普萘洛尔的给药未影响蜂蜜的效果。六甲铵在试验的早期和晚期也未影响蜂蜜的效果，而阿托品仅抑制试验的早期阶段。然而，阿托品和六甲铵未影响蜂蜜的抗炎作用，但坦索罗辛消除了这些作用，而普萘洛尔仅在炎症高峰期消除了抗炎作用。结果表明自主神经受体参与了蜂蜜的抗伤害感受和抗炎作用，尽管参与程度取决于不同类型的受体。
