United States Department of Agriculture, Agricultural Research Service, Box 7631, 27695-7631, Raleigh, NC, USA.
Photosynth Res. 1993 May;36(2):103-10. doi: 10.1007/BF00016275.
Plastocyanin levels in barley (Hordeum vulgare cv Boone) were found to be dependent on growth irradiance. An immunochemical assay was developed and used to measure the plastocyanin content of isolated thylakoid membranes. Barley grown under 600 μmole photons m(-2)s(-1) contained two- to four-fold greater quantities of plastocyanin per unit chlorophyll compared with plants grown under 60 μmole photons m(-2)s(-1). The plastocyanin/Photosystem I ratio was found to be 2 to 3 under high irradiance compared with 0.5 to 1.5 under low irradiance. The reduced plastocyanin pool size in low light plants contributed to a two-fold reduction in photosynthetic electron transport activity. Plastocyanin levels increased upon transfer of low light plants to high irradiance conditions. In contrast, plastocyanin levels were not affected in plants transferred from high to low irradiance, suggesting that plastocyanin is not involved in the acclimation of photosynthesis to shade.
大麦（Hordeum vulgare cv Boone）的质体蓝素水平被发现依赖于生长光强。开发了一种免疫化学测定法，并用于测量分离的类囊体膜中的质体蓝素含量。与在 60 μmole photons m(-2)s(-1)下生长的植物相比，在 600 μmole photons m(-2)s(-1)下生长的大麦每单位叶绿素中的质体蓝素含量增加了两到四倍。在高光强下，质体蓝素/光系统 I 的比例为 2 到 3，而在低光强下为 0.5 到 1.5。在低光下，质体蓝素池的大小减小导致光合作用电子传递活性降低了两倍。当将低光植物转移到高光强条件下时，质体蓝素水平会增加。相比之下，当将植物从高光强转移到低光强条件下时，质体蓝素水平没有受到影响，这表明质体蓝素不参与光合作用对阴凉的适应。
