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通过酶联免疫斑点分析对人类记忆性T细胞进行计数和表征。

Enumeration and characterization of human memory T cells by enzyme-linked immunospot assays.

作者信息

Calarota Sandra A, Baldanti Fausto

机构信息

S. S. Virologia Molecolare, S. C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Via Taramelli 5, 27100 Pavia, Italy.

出版信息

Clin Dev Immunol. 2013;2013:637649. doi: 10.1155/2013/637649. Epub 2013 Nov 11.

DOI:10.1155/2013/637649
PMID:24319467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3844203/
Abstract

The enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole antigens, total lymphocyte or isolated T-cell subset responses can be assessed either after short-term stimulation (standard ELISPOT) or after their expansion during a 10-day culture (cultured ELISPOT). Both assays detect different antigen-specific immune responses allowing the analysis of effector memory T cells and central memory T cells. This paper describes the principle of ELISPOT assays and discusses their application in the evaluation of immune correlates of clinical interest with a focus on the vaccine field.

摘要

酶联免疫斑点（ELISPOT）检测已发展成为一种有用且广泛应用的工具，用于评估人类和疾病动物模型及/或候选疫苗中的T细胞反应。使用合成肽（单独或作为重叠肽混合物）或全抗原，可在短期刺激后（标准ELISPOT）或在10天培养期间细胞扩增后（培养ELISPOT）评估总淋巴细胞或分离的T细胞亚群反应。两种检测方法均可检测不同的抗原特异性免疫反应，从而分析效应记忆T细胞和中枢记忆T细胞。本文描述了ELISPOT检测的原理，并讨论了其在评估具有临床意义的免疫相关性方面的应用，重点是疫苗领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7ba/3844203/0ec72c466ece/CDI2013-637649.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7ba/3844203/4d8cd7114f45/CDI2013-637649.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7ba/3844203/0ec72c466ece/CDI2013-637649.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7ba/3844203/4d8cd7114f45/CDI2013-637649.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7ba/3844203/0ec72c466ece/CDI2013-637649.002.jpg

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