Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China ; Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
Evid Based Complement Alternat Med. 2013;2013:572078. doi: 10.1155/2013/572078. Epub 2013 Nov 11.
Objective. We investigated the effects of WXKL on the action potential (AP) and the L-type calcium current (I Ca-L) in normal and hypertrophied myocytes. Methods. Forty male rats were randomly divided into two groups: the control group and the transverse aortic constriction- (TAC-) induced heart failure group. Cardiac hypertrophy was induced by TAC surgery, whereas the control group underwent a sham operation. Eight weeks after surgery, single cardiac ventricular myocytes were isolated from the hearts of the rats. The APs and I Ca-L were recorded using the whole-cell patch clamp technique. Results. The action potential duration (APD) of the TAC group was prolonged compared with the control group and was markedly shortened by WXKL treatment in a dose-dependent manner. The current densities of the I Ca-L in the TAC group treated with 5 g/L WXKL were significantly decreased compared with the TAC group. We also determined the effect of WXKL on the gating mechanism of the I Ca-L in the TAC group. We found that WXKL decreased the I Ca-L by accelerating the inactivation of the channels and delaying the recovery time from inactivation. Conclusions. The results suggest that WXKL affects the AP and blocked the I Ca-L, which ultimately resulted in the treatment of arrhythmias.
目的。本研究旨在探讨WKXL 对正常和肥大心肌细胞动作电位（AP）和 L 型钙电流（ICa-L）的影响。
方法。40 只雄性大鼠随机分为两组：对照组和主动脉缩窄（TAC）诱导的心力衰竭组。TAC 手术后诱导心肌肥厚，而对照组则进行假手术。手术后 8 周，从大鼠心脏中分离出单个心室肌细胞。使用全细胞膜片钳技术记录 AP 和 ICa-L。
结果。与对照组相比，TAC 组的动作电位时程（APD）延长，而 WXKL 处理呈剂量依赖性显著缩短。与 TAC 组相比，用 5 g/L WXKL 处理的 TAC 组的 ICa-L 电流密度显著降低。我们还确定了 WXKL 对 TAC 组 ICa-L 门控机制的影响。结果发现，WXKL 通过加速通道失活和延迟失活恢复时间来减少 ICa-L。
结论。结果表明，WXKL 影响 AP 并阻断 ICa-L，最终导致心律失常的治疗。
