对阻断针对M315的迟发型超敏反应的独特型特异性抑制因子的精细特异性分析：支持M315的λ2链可变区体细胞突变起作用的证据。
Fine specificity analysis of idiotype-specific suppressor factors that block a delayed-type hypersensitivity response to M315: evidence supporting a role for somatic mutations in the variable region of the lambda 2 chain of M315.
作者信息
Sakato N, Azuma T, Fujio H
出版信息
Immunology. 1986 Nov;59(3):365-72.
The delayed-type hypersensitivity (DTH) response in BALB/c mice to the idiotype of M315 (alpha,lambda 2), a BALB/c myeloma protein, can be suppressed by a single i.v. injection of soluble M315. This suppression involves the generation of suppressor T cells. Mice tolerized by M315 produce subcellular factors that suppress the M315-DTH response. Such suppressor factors can be obtained by mechanical disruption of spleen cells or thymocytes from mice treated i.v. with M315. The fine specificity of the factor was studied using various immunoadsorbents such as L315 and germline V lambda 2 chain-Sepharose beads with known amino acid sequences. The results indicated that the specificity of the factor depended on three contiguous somatically mutated amino acid residues, Phe94, Arg95 and Asn96, in the third hypervariable region of the V domain of the L chain of M315.
BALB/c小鼠对BALB/c骨髓瘤蛋白M315（α，λ2）独特型的迟发型超敏反应（DTH），可通过静脉内单次注射可溶性M315来抑制。这种抑制涉及抑制性T细胞的产生。经M315耐受的小鼠产生可抑制M315-DTH反应的亚细胞因子。此类抑制因子可通过机械破碎经静脉注射M315处理的小鼠的脾细胞或胸腺细胞获得。使用各种免疫吸附剂，如具有已知氨基酸序列的L315和种系Vλ2链-琼脂糖珠，研究了该因子的精细特异性。结果表明，该因子的特异性取决于M315轻链V结构域第三个高变区中三个相邻的体细胞突变氨基酸残基，即苯丙氨酸94、精氨酸95和天冬酰胺96。
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