一种针对沙门氏菌脂多糖外核心寡糖的鼠单克隆抗体。
A murine monoclonal antibody specific for the outer core oligosaccharide of Salmonella lipopolysaccharide.
作者信息
Tsang R S, Chan K H, Chau P Y, Wan K C, Ng M H, Schlecht S
出版信息
Infect Immun. 1987 Jan;55(1):211-6. doi: 10.1128/iai.55.1.211-216.1987.
Abstract
Immunoglobulin G3 murine monoclonal antibody T6 specific for the lipopolysaccharide of Salmonella O serogroups A to E was established. By using R mutants of Salmonella spp., Escherichia coli, and Shigella spp., the major reactive epitope with T6 was tentatively identified as the terminal disaccharide, N-acetylglucosamine 1.2----alpha glucose, of the core oligosaccharide. T6 was reactive with 10 clinical isolates of each of the Salmonella O serogroups A to E but not with 58 isolates of other gram-negative bacteria. Its selective reactivity against Salmonella spp. renders T6 a potentially more useful reagent than the conventional polyvalent serum for the identification of Salmonella spp. It may also serve as a useful molecular tool for the study of the outer core structure of all Salmonella and related species.
摘要
建立了对沙门氏菌O血清群A至E的脂多糖具有特异性的免疫球蛋白G3鼠单克隆抗体T6。通过使用沙门氏菌属、大肠杆菌属和志贺氏菌属的R突变体，初步确定与T6的主要反应表位为核心寡糖的末端二糖，N-乙酰葡糖胺1.2----α葡萄糖。T6与沙门氏菌O血清群A至E的各10株临床分离株有反应，但与58株其他革兰氏阴性菌无反应。它对沙门氏菌属的选择性反应使T6成为比传统多价血清更有用的试剂，用于沙门氏菌属的鉴定。它也可作为研究所有沙门氏菌和相关菌种外核心结构的有用分子工具。
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