Kasaian Jamal, Iranshahy Milad, Masullo Milena, Piacente Sonia, Ebrahimi Fatemeh, Iranshahi Mehrdad
a Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran.
J Asian Nat Prod Res. 2014;16(3):248-53. doi: 10.1080/10286020.2013.866099. Epub 2013 Dec 10.
Two new sesquiterpene lactones, namely feruhodin A and feruhodin B, together with six known compounds, daucoeudesmanolactone, dehydrooopodin, oopodin, badkhysin, 7-demethylplastochromenol, and scoparone, were isolated from the roots of Ferula oopoda. The structures of these compounds were elucidated by 1D and 2D NMR techniques as well as high-resolution mass spectrometry. Cytotoxic effects of these compounds were evaluated against two human cancer cell lines including MCF7and K562 using the Alamar blue assay. The results showed that dehydrooopodin possessed significant cytotoxic effects with IC₅₀ values of 15 and 5 μM against MCF7 and K562, respectively.
从欧防风（Ferula oopoda）的根中分离出两种新的倍半萜内酯，即阿魏奥多辛A（feruhodin A）和阿魏奥多辛B（feruhodin B），以及六种已知化合物，即胡萝卜欧防风内酯（daucoeudesmanolactone）、脱氢奥波定（dehydrooopodin）、奥波定（oopodin）、巴德辛（badkhysin）、7-去甲基质体色素酚（7-demethylplastochromenol）和滨蒿内酯（scoparone）。通过一维和二维核磁共振技术以及高分辨率质谱对这些化合物的结构进行了阐明。使用阿拉玛蓝测定法评估了这些化合物对包括MCF7和K562在内的两种人类癌细胞系的细胞毒性作用。结果表明，脱氢奥波定具有显著的细胞毒性作用，对MCF7和K562的IC₅₀值分别为15 μM和5 μM。
