Jaremicz Zbigniew, Luczkiewicz Maria, Kokotkiewicz Adam, Krolicka Aleksandra, Sowinski Pawel
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland,
Biotechnol Lett. 2014 Apr;36(4):843-53. doi: 10.1007/s10529-013-1426-9. Epub 2013 Dec 10.
Hairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production. The latter gave the highest anisodamine content (0.67 mg/g dry wt) whereas scopolamine, hyoscyamine and cuscohygrine concentrations were highest in the bubble-column reactor (5.3, 1.6 and 26.5 mg/g dry wt, respectively). Both bioreactors gave similar productivities of scopolamine (1 and 0.98 mg/l day) and cuscohygrine (5 and 5.4 mg/l day), but anisodamine productivity was 3.5-fold higher in the hybrid bioreactor (HB) (0.02 and 0.07 mg/l day, respectively). Elicitation with methyl jasmonate increased scopolamine productivity by 146 % in roots grown in the HB whereas their permeabilization with DMSO caused 4-, 5-, 25- and 28-fold increase in scopolamine, hyoscyamine, anisodamine and cuscohygrine concentrations in the growth medium. In situ extraction with Amberlite XAD-2 doubled scopolamine productivity in the hybrid reactor after 50 days.
将黑莨菪的毛状根培养物在摇瓶、鼓泡塔生物反应器和鼓泡塔/喷雾混合生物反应器中进行培养，并对生物碱产量进行评估。后者的山莨菪碱含量最高（0.67毫克/克干重），而东莨菪碱、莨菪碱和古柯碱的浓度在鼓泡塔反应器中最高（分别为5.3、1.6和26.5毫克/克干重）。两种生物反应器中东莨菪碱（1和0.98毫克/升·天）和古柯碱（5和5.4毫克/升·天）的生产率相似，但混合生物反应器（HB）中山莨菪碱的生产率高出3.5倍（分别为0.02和0.07毫克/升·天）。用茉莉酸甲酯诱导使在HB中生长的根中东莨菪碱的生产率提高了146%，而用二甲基亚砜使其透化导致生长培养基中东莨菪碱、莨菪碱、山莨菪碱和古柯碱的浓度分别增加了4倍、5倍、25倍和28倍。在混合反应器中用Amberlite XAD - 2进行原位萃取50天后，东莨菪碱的生产率提高了一倍。
