1977年至2008年从巴西牛群中分离出的流产布鲁氏菌的生物分型和基因分型（MLVA16）
Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008.
作者信息
Minharro Sílvia, Silva Mol Juliana P, Dorneles Elaine M S, Pauletti Rebeca B, Neubauer Heinrich, Melzer Falk, Poester Fernando P, Dasso Maurício G, Pinheiro Elaine S, Soares Filho Paulo M, Santos Renato L, Heinemann Marcos B, Lage Andrey P
机构信息
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil ; Escola de Medicina Veterinária e Zootecnia, Universidade Federal do Tocantins, Araguaína, Tocantins, Brazil.
出版信息
PLoS One. 2013 Dec 6;8(12):e81152. doi: 10.1371/journal.pone.0081152. eCollection 2013.
Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes of Brucella spp. is scarce or unavailable. This study aimed (i) to biotype and genotype 137 Brazilian cattle isolates (from 1977 to 2008) of B. abortus and (ii) to analyze their distribution. B. abortus biovars 1, 2 and 3 (subgroup 3b) were confirmed and biovars 4 and 6 were first described in Brazil. Genotyping by the panel 1 revealed two groups, one clustering around genotype 40 and another around genotype 28. Panels 2A and 2B disclosed a high diversity among Brazilian B. abortus strains. Eighty-nine genotypes were found by MLVA16. MLVA16 panel 1 and 2 showed geographic clustering of some genotypes. Biotyping and MLVA16 genotyping of Brazilian B. abortus isolates were useful to better understand the epidemiology of bovine brucellosis in the region.
布鲁氏菌病是一种分布于全球的人畜共患病，给动物生产造成重大经济损失。在巴西，关于布鲁氏菌生物型和基因型分布的信息匮乏或难以获取。本研究旨在：（i）对137株巴西牛源流产布鲁氏菌分离株（1977年至2008年）进行生物分型和基因分型，以及（ii）分析它们的分布情况。确认了流产布鲁氏菌生物型1、2和3（3b亚群），生物型4和6首次在巴西被描述。通过第1组基因分型揭示了两个组，一组聚集在基因型40周围，另一组围绕基因型28。第2A组和2B组揭示了巴西流产布鲁氏菌菌株间的高度多样性。通过MLVA16发现了89种基因型。MLVA16第1组和第2组显示出一些基因型的地理聚集现象。巴西流产布鲁氏菌分离株的生物分型和MLVA16基因分型有助于更好地了解该地区牛布鲁氏菌病的流行病学情况。
Zotero 插件