Yu G S, Steinkirchner T M, Rao G A, Larkin E C
Am J Pathol. 1986 Dec;125(3):620-4.
In this study, a histopathologic examination of the brain from iron-deficient or iron-supplemented rat pups was carried out. Pups were obtained from female rats, which were fed an iron-deficient or iron-supplemented diet during both pregnancy and lactation. Immediately after anesthesia and the collection of blood, pups were fixed by intracardiac infusion of 2% glutaraldehyde. Brain and cervical spinal cord were fixed, embedded in paraffin, and cut at 6-mu thickness. Myelin was identified using Luxol fast blue stain. As compared with controls (hematocrit, 30.8%), 11-day-old iron-deficient pups (hematocrit, 11.9%) showed reduced myelination in the spinal cord. Although myelination increased somewhat in the iron-deficient 17-day-old pups (hematocrit, 8.5%), the amount of myelin in the spinal cord and white matter of cerebellar folds was reduced as compared with that of the corresponding controls. These observations show the importance of prenatal iron adequacy in myelinogenesis.
在本研究中，对缺铁或补铁的幼鼠大脑进行了组织病理学检查。幼鼠取自雌性大鼠，这些雌性大鼠在怀孕和哺乳期均喂食缺铁或补铁饮食。在麻醉和采血后，立即通过心脏内注入2%戊二醛对幼鼠进行固定。将大脑和颈脊髓固定、石蜡包埋，并切成6微米厚的切片。使用Luxol固蓝染色鉴定髓磷脂。与对照组（血细胞比容为30.8%）相比，11日龄缺铁幼鼠（血细胞比容为11.9%）脊髓中的髓鞘形成减少。尽管17日龄缺铁幼鼠（血细胞比容为8.5%）的髓鞘形成有所增加，但与相应对照组相比，其脊髓和小脑皱襞白质中的髓磷脂量减少。这些观察结果表明产前铁充足在髓鞘形成中的重要性。
